Genomic characterization of a porcine sapelovirus isolated from mosquitoes in Shandong province,China
Objective The whole genome of one porcine sapelovirus(PSV)strain from mosquitoes captured in Weishan county,Shandong province was sequenced and analyzed its genetic characteristics.Methods Mosquitoes collected in pigpen in Weishan county of Shandong province in 2021 were homogenized and inoculated on C6/36 and BHK21 cell lines for virus isolation.Virus RNA was extracted from the positive culture and the whole genome was sequenced by next generation sequencing.Phylogenetic analysis,similarity comparison and recombination analysis were conducted via MEGA 7.0.26,BioEdit 7.0.9.0 and Simplot 3.5.1.Results A PSV strain 265/SD was isolated from Culex tritaeniorhynchus.The clear cytopathic effects characterized by rounding,shrinkage and detachment of cells were observed on BHK21 cells.The whole genome of this study strain was 7 558 nt in length.Phylogenetic analysis based on VP1 sequences indicated that strain 265/SD belonged to traditional genotype.Different positions of 265/SD in the VP1 and 3D phylogenetic trees suggested the recombinant potential.Similarity plot revealed the existence of recombination but the exact origins remained unknown.The similarity analysis of VP1 regions showed that strain 265/SD had low nucleotide identity(77.3％)and amino acid identity(80.3％)with another strain SD/CHN/2016 from Shandong province.Conclusions Our data enriched the whole genome information of PSV in Shandong province and provided scientific basis for prevention and control of PSV.
万方数据
维普
