Objective To establish a rat model of type 2 diabetes mellitus treated by Roux-en-Y gastric bypass operation.Methods Forty adult SD rats were randomly divided into two groups:model group (n =30) and normal control group (NC,n =10).Model group rats were subjected to streptozotocin (STZ) intraperitoneal injection 4 weeks after feeding on high-fat and high-sugar diet.Rat model of type 2 diabetes mellitus was established successfully after detection of increased blood glucose,cholesterol and triglyceride in model group as compared with those in control group.Twenty-eight rats with type 2 diabetes mellitus were randomly divided into 3 groups:Roux-en-Y gastric bypass operation group (RYGB,n =10),sham operation group (SO,n =10),no operation group (NO,n =8).Results The blood glucose (＞ 16.7 mmol/L averagely),cholesterol and triglyceride levels were increased obviously in model group as compared with those in control group.Meanwhile,"eat more,drink more,urine more and weight loss" occurred in model group,suggesting the successful establishment of rat model of type 2 diabetes mellitus.The blood glucose,cholesterol and triglyceride levels in RYGB group were decreased at first week after operation,and nearly reduced to normal level at 4th week after operation.The overall survival rate in RYGB group was increased to 80％ by improved operation technique and enhanced perioperative management.All rats in 4 groups were alived at 8th week post-operation.Condusion This improved technique is feasible for establishment of rat model of type 2 diabetes mellitus treated by Roux-en-Y bypass operation.This model can be observed and studied for long time,and will contribute to evaluation of long-term effect of operation on type 2 diabetes mellitus and give more help in mechanism research.
Type 2 diabetes mellitusRou-xen-Y gastric bypass operationModel, animal
NETL
NSTL
维普
万方数据
