Expression of DNA methyltransferase 3A in pancreatic cancer and screening of its regulatory factors
Objective To investigate the expression level of DNA methyltransferase 3A in pancre-atic cancer tissues and adjacent tissues,and to screen the microRNA(miRNA,miR)family that regulates DNA methyltransferase 3A by transcriptomics.Methods From June 2021 to June 2023,the pancreatic cancer tissues and corresponding adjacent tissues resected in the inpatient department of the First Affiliated Hospital of Xinxiang Medical College were selected as the research objects.The expression level of DNA methyltransferase 3A was analyzed by Western blotting.A total of 6 cases of adjacent tissues and pancreatic cancer tissues were randomly selected as research objects.The differential expression(microRNA,miRNA)in adjacent tissues and pancreatic cancer tissues was analyzed by transcriptomics,and the regula-tion relationship of differential expression genes to DNA methyltransferase 3A was analyzed by bioinformat-ics and luciferase reporter genes.The comparison of quantitative data was done by t-test.Results The expression level of DNA methyltransferase 3A in adjacent tissues(0.77±0.16)was significantly lower than that in pancreatic cancer tissues(1.48±0.21,t=19.690,P＜0.05).There were 390 differentially expressed miRNAs in pancreatic cancer tissues and adjacent tissues,of which 259 were up-regulated and 131 were down-regulated.The main biological processes of these miRNAs included cell proliferation,cell development,cell differentiation.Molecular functions mainly included tumor cell movement,cell migra-tion,cell invasion,cell proliferation,and intercellular signal transduction.It was found that downregulated miRNAs were complementary to DNA methyltransferase 3A,with the top 10 miRNAs with the most signifi-cant differences being miR-143-3p,miR-129-5p,miR-493-5p,miR-367-3p,miR-29a-3p,miR-212-3p,miR-323a-3p,miR-5195-3p,miR-465,and miR-532-5p,respectively.The expression levels of miR-143-3p,miR-129-5p,miR-493-5p,miR-367-3p,miR-29a-3p,miR-212-3p,miR-323a-3p,miR-5195-3p,miR-4465 and miR-532-5p in adjacent cancerous tissues(1.09±0.11,0.97±0.08,1.08±0.31,1.17±0.121,1.07±0.08,1.01±0.10,0.97±0.08,1.01±0.05,0.99±0.03,1.04±0.10)were significantly higher than those of pancreatic cancer tissues(0.51±0.10,0.46±0.11,0.47±0.15,0.62±0.12,0.37±0.07,0.56±0.10,0.66±0.04,0.68±0.06,0.73±0.05,0.66±0.13,t=11.860,12.170,5.590,9.910,21.070,9.941,11.180,14.030,13.570,7.470,P＜0.05).The luciferase activity of cells co-transfected with miRNA control and luciferase reporter genes(0.97±0.02,0.96±0.02,0.97±0.02,0.98±0.02,0.97±0.02,0.97±0.04,0.95±0.03,0.96±0.03,0.97±0.02,0.96±0.02)was significantly higher than that of miR-143-3p,miR-129-5p,miR-493-5p,miR-367-3p,miR-29a-3p,miR-212-3p,miR-323a-3p,miR-5195-3p miR-4465 and miR-532-5p mimetics wwith luciferase reporter(0.32±0.07;0.33±0.06;0.35±0.09;0.50±0.04;0.50±0.05;0.53±0.07;0.58±0.03;0.63±0.03;0.65±0.05;0.81±0.05,t=22.400,24.470,16.730,24.480,20.530,14.010,21.360,19.040,15.960,6.751,P＜0.05).Conclusion The expression level of DNA methyltransferase 3a in pancreatic cancer tissue is significantly increased.miR-143-3p,miR-129-5p,miR-493-5p,miR-367-3p,miR-29a-3p,miR-212-3p,miR-323a-3p,miR-5195-3p,miR-4465 and miR-532-5p are involved in regulating the expression of DNA methyltrans-ferase 3A in pancreatic cancer tissue.
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