Impact of long non coding RNA linc00921 on cell invasion ability in triple-negative breast cancer via targeted regulation of microRNA-9-5p
Objective To observe the impact of long non-coding RNA linc00921 in triple-negative breast cancer cells on cell invasion ability through targeted regulation of microRNA(miR)-9-5p.Methods A total of 30 samples,including triple-negative breast cancer and adjacent tissues,from the Department of Breast Surgery at the Fourth Hospital of Hebei Medical University,collected between April 2019 and April 2020,were utilized as the research subjects.The correlation between the expression of linc00921 and miR-9-5p was analyzed using real-time fluorescence quantitative polymerase chain reaction(PCR).The MDA-MB-231 cells were divided into linc00921 overexpression group and control group,and the impact on cell invasion ability was observed through a cell invasion experiment.The effect oflinc00921 on the expres-sion of miR-9-5p was detected using real-time fluorescence quantitative PCR.Bioinformatics analysis and dual-luciferase reporter gene assay were employed to verify the relationship between linc00921 and miR-9-5p.The miR-9-5p mimics+linc00921 group and miR-NC+linc00921 group were set up,and the impact of linc00921 on cell invasion ability was observed to determine its relationship with miR-9-5p.T-test was per-formed to compare the two groups.Results The number of invasive cells in the linc00921 overexpression group(154.50±12.06)was significantly less than that in the control group(400.50±6.36,t=61.500,P＜0.05).The bioinformatics analysis and dual luciferase reporter gene validation results indicated that linc00921 could bind to miR-9-5p and reduce the luciferase activity in MDA-MB-231 cells(0.41±0.04 vs.1.03±0.09,t=17.570,P＜0.05).The correlation analysis results revealed a negative correlation between linc00921 and miR-9-5p expression levels(r=-0.484,P＜0.05).The rescue experiment re-sults demonstrated that the number of invasive cells in the linc00921+miR-9-5p mimics group was signifi-cantly greater than that in the linc00921+miR-NC group[(335.50±14.84)vs.(122.50±4.94),t=30.430,P＜0.05].Conclusion The long non-coding RNA linc00921 can affect the invasive ability of triple-negative breast cancer cells by targeting and regulating miR-9-5p.
Triple negative breast cancerlinc00921MicroRNA-9-5pInvasion
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