The expression changes of cylindromatosis in non-small cell lung cancer and its impact on prolifera-tion and metastasis characteristics
Objective To investigate the expression changes of cylindromatosis(CYLD)gene in non-small cell lung cancer(NSCLC)and its impact on proliferation and metastasis characteristics.Methods Totally,93 cases of NSCLS tissues and adjacent tissues admitted to Fuwai Huazhong Cardiovas-cular Hospital,Henan Provincial People's Hospital,Zhengzhou University People's Hospital from June 2021 to December 2023 were selected as the research subjects.The expression level of CYLD mRNA in the tissues was analyzed using fluorescence quantitative polymerase chain reaction(PCR).The human NSCLC H1299 cells were infected with control lentivirus and CYLD overexpression lentivirus to establish a control group and CYLD group.The effect of CYLD on cell proliferation was analyzed using cell counting kit-8(CCK-8),EdU,and in vitro tumor transplantation.The effect of CYLD on the metastasis of NSCLC was analyzed using scratch test,Transwell test,and Western blotting.Western blotting analysis was used to in-vestigate the effect of CYLD on the expression levels of proliferation cell nuclear antigen(Ki-67),c-MYC,Cyclin D1,c-Jun N-terminal kinase(JNK),and activation factor protein 1(AP1)proteins in cells.The comparison of inter group measurement data was done by t-test.Results The expression level of CLYD protein in adjacent cancer tissues(1.02±0.17)was significantly higher than that in non-small cell lung cancer tissues(0.49±0.29,t=15.300,P＜0.05).The expression level of CLYD mRNA in adjacent tis-sues(1.52±0.23)was significantly higher than that in non-small cell lung cancer tissues(0.68±0.12,t=30.860,P＜0.05).The cell viability of the control group[(86.84±6.05)％]was significantly higher than that of the GLYD group[(57.95±3.79)％,t=9.909,P＜0.05].The positive rate of EdU in the control group[(82.97±5.65)％]was significantly higher than that in the GLYD group[(49.95± 9.43)％,t=7.352,P＜0.05].The tumor formation volume in the control group[(919.09±61.86)mm3]was significantly greater than that in the GLYD group[(639.93±51.24)mm3,t=8.512,P＜0.05].The scratch healing rate in the control group[(83.49±4.20)％]was significantly higher than that in the GLYD group[(46.71±5.15)％,t=13.560,P＜0.05].The scratch healing rate in the control group[(141.83±11.48)cells]was significantly higher than that in the GLYD group[(87.17±7.70)cells,t=9.686,P＜0.05].The number of metastatic foci in the control group(11.17±2.79)was significantly greater than that in the GLYD group(5.67±2.07,t=3.884,P＜0.05).The expression levels of Ki-67,c-MYC,Cyclin D1,JNK and AP1 proteins in the control group(0.95±0.10,1.20±0.06,0.93±0.06,1.48±0.09,1.31±0.08)were significantly higher than those in the GLYD group(0.63±0.07,0.81± 0.10,0.67±0.12,1.04±0.13,0.63±0.07,t=6.612,9.613,4.657,6.724,15.770,P＜0.05).Conclusion CYLD is down-expressed in NSCLC,and overexpression of CYLD can inhibit the prolifera-tion and metastasis of NSCLC,mainly related to JNK1 signaling pathway.
Cylindrical tumor geneNon small cell lung cancerProliferationMigrationC-Jun N-terminal kinase pathway
NETL
NSTL
万方数据
