Expression and clinical significance of high mobility group box 1 and autophagy associated protein p62 in colon carcinoma tissues
Objective To investigate the clinical significance of high mobility group box 1(HMGB1)and autophagy related protein p62 expression in colon carcinoma tissues,as well as evaluate the relationship between the clinicopathological features and prognosis of colon carcinoma and these proteins.Methods The expression levels of HMGB1 and p62 in 108 cases of colon carcinoma tissues and adjacent colon tissues pathologically confirmed from January 2015 to December 2017 on Wuhan Fourth Hospital were examined by immunohistochemical staining.Spearman rank correlation test was used to analyze the rela-tionship between HMGB1 and p62 expression.The relationship between the expression of HMGB1 and p62 and clinicopathological parameters was evaluated by Chi-square test.Kaplan-Meier method and Log-Rank test were used for survival analysis,and COX regression model was used for multivariate analysis.Results The expression rate of HMGB1 and p62 in colon carcinoma tissues was 70.37％(76/108)and 80.56％(87/108),respectively,and there was a positive correlation between them(rs=0.450,P＜0.01).The expression of HMGB1 was significantly correlated with the histological type and lymph node metastasis of colon carcinoma(respectively,x2=6.704,12.725,P＜0.05).The expression of p62 was significantly correlated with lymph node metastasis of colon carcinoma(x2=8.865,P＜0.05).Kaplan-Meier survival analysis showed that the median progression-free survival(PFS)[(32.0±4.84)months]in HMGB1-posi-tive patients was significantly shorter than that in HMGB1-negative patients(x2=11.805,P＜0.01).The median PFS of p62 positive patients[(36.0±4.12)months]was significantly shorter than that of negative patients(x2=11.805,P＜0.01).Multivariate regression analysis showed that HMGB1[hazard ratio(HR)=2.406,95％confidence interval(CI):1.053-5.493,P＜0.05]and p62(HR=6.275,95％CI:1.518-25.941,P＜0.05)were independent risk factors affecting the PFS of colon carcinoma patients.Conclusion HMGB1 and p62 are highly expressed in colon carcinoma tissues.The combined detection of HMGB1 and p62 may be of value in evaluating the biological behavior and prognosis of colon carcinoma.
High mobility group box 1Autophagy associated protein p62Colon carcinomaAutophagy
NETL
NSTL
万方数据
