CCAAT/enhancer binding protein p as a potential biomarker for predicting the prognosis of gastric cancer
Objective To explore the potential prognostic value of CCAAT/enhancer-binding pro-tein beta(CEBPB)in gastric cancer.Methods CEBPB-related expression profiles and relevant clinical data were downloaded from the Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)data-sets to validate the expression of CEBPB in gastric cancer cell lines.Gene Set Enrichment Analysis(GSEA)was employed to identify CEBPB-related pathways in gastric cancer.The correlation between CEBPB expression and clinical features in gastric cancer was assessed through statistical analysis.The spe-cific functions of CEBPB in gastric cancer were explored through cell proliferation experiments(cell count-ing kit-8,CCK-8),cell scratch assays,and colony formation assays.Statistical analyses were performed u-sing SPSS 26.0 and GraphPad Prism 7.Results CEBPB expression in gastric cancer tissues was signifi-cantly higher than in non-tumor tissues(2 870.0±4 485.0 vs.908.1±957.6,t=6.264,P＜0.05),and CEBPB expression in gastric cancer cell lines(AGS,MKN-45,HGC-27)was higher than in GES-1 cells.Furthermore,patients with high CEBPB expression had significantly lower average overall survival(OS)and first progression(FP)than low-expression patients.GSEA revealed associations between CEBPB and various signaling pathways and biological processes related to tumor progression(PID SMAD23NUCLEAR pathway,apoptosis negative regulation signaling pathway,VEGFA-VEGFR2 signaling path-way,IL-4 signaling pathway,RNA biosynthetic process,etc.).Protein network analysis of CEBPB sug-gested interactions with ATF4,CEBPA,CEBPD,DDIT3,EP300,HDAC1,MYB,PPARG,PRDM16,and STAT3.CCK-8 assays showed that cell proliferation in the CEBPB knockdown group was lower than in the control group(0.51±0.05 vs.0.68±0.17,0.32±0.05 vs.0.68±0.17,t=2.950,6.280,P＜0.05).Cloning experiments demonstrated that the CEBPB knockdown group had fewer colonies than the control group(148.0±60.1 vs.681.0±156.5,491.0±175.0 vs.681.0±156.5,t=1.400,5.510,P＜0.05),and scratch assays showed that the migration rate of the CEBPB knockdown group was signifi-cantly higher than the control group(0.86±0.07 vs.0.71±0.04,0.87±0.03 vs.0.71±0.04,t=5.110,8.920,P＜0.05).Conclusion The study revealed that CEBPB plays an oncogenic role in gas-tric carcinoma and may serve as a clinical prognostic factor for gastric carcinoma.
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