Effects of long non-coding RNA GAS5 targeting microRNA-106b-5p on proliferation,migration and invasion of gastric cancer cells
Objective To observe the effects of long non-coding RNA(lncRNA)GAS5 targeting microRNA(miR)-106b-5p on proliferation,migration and invasion of gastric cancer cells.Methods A total of 112 gastric cancer tissues and adjacent tissues were selected as the research objects.The expression levels of lncRNA GAS5 and miR-106b-5p in tissues were detected by fluorescence quantitative polymerase chain re-action(PCR).HGC-27 cells were randomly divided into lncRNA control group,lncRNA GAS5 group,miRNA control group and miR-106b-5p KD group.LncRNA GAS5 overexpression and miR-106b-5p knock-down cell lines were established by lentivirus,respectively.Cell counting kit-8(CCK-8),scratch and Tran-swell assays were used to analyze cell proliferation,migration and invasion in each group,respectively.The relationship between lncRNA GAS5 and miR-106b-5p was analyzed by bioinformatics and dual lucifer-ase reporter gene.T-test was used for inter group comparison.Results The expression level of lncRNA GAS5 in paracancer tissues(1.003±0.107)was significantly lower than that in gastric cancer tissues(0.387±0.060,t=8.649,P＜0.05).The expression level of lncRNA GAS5 in lncRNA GAS5 group(3.073±0.373)was significantly higher than that in lncRNA control group(1.007±0.097,t=9.284,P＜0.05).The expression level of miR-106b-5p in miR-106b-5p knockout group(0.333±0.283)was significantly lower than that in miRNA control group(1.123±0.076,t=11.880,P＜0.05).The absor-bance value of lncRNA GAS5 group(1.453±0.090)was significantly lower than that of lncRNA control group(1.983±0.196,t=4.254,P＜0.05).The absorbance of miR-106b-5p knockout group(1.277± 0.081)was significantly lower than that of miRNA control group(2.083±0.191,t=6.781,P＜0.05).The scratch healing rate of lncRNA GAS5 group[(43.347±4.713)％]was significantly lower than that of lncRNA control group[(79.643±7.069)％,t=7.399,P＜0.05).The scratch healing rate of miR-106b-5p knockout group[(39.773±1.817)％]was significantly lower than that of miRNA control group[(82.547±2.811)％,t=18.633,P＜0.05].The number of invasive cells in lncRNA GAS5 group[(58.137±5.167)]was significantly less than that in lncRNA control group[(89.733±8.942),t=5.128,P＜0.05].The number of invasive cells in miR-106b-5p knockout group[(51.917±2.671)]was significantly less than that in miRNA control group[(92.473±6.209),t=10.393,P＜0.05].LncRNA GAS5 and miR-106b-5p had base complementary.The expression level of miR-106b-5p in ln-cRNA GAS5 group(0.563±0.067)was significantly lower than that in lncRNA control group(1.187± 0.100,t=8.976,P＜0.05).Conclusion lncRNA GAS5 expression is up-regulated in gastric cancer tissues,and the expression of miR-106b-5p is down-regulated.LncRNA GAS5 regulates the proliferation,migration and invasion of gastric cancer cells through competitive adsorption and binding to miR-106b-5p.
Long non-coding RNAMicroRNAGastric cancerProliferationMigration
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