Expression of G protein signaling regulatory factor 17 in lung cancer and its impact on cell prolifer-ation and metastasis
Objective To investigate the expression of G protein signaling regulatory factor 17 in lung cancer cells and its effects on cell proliferation and metastasis.Methods The expression levels of G protein signaling regulator 17 protein in human normal lung epithelial cells(BEAS-2B)and lung cancer cells(A549,H1299,H446,and H1703)was analyzed by Western blotting.By American Center for the Collection ofTypical Strains(ATCC)Corporation in the United States,H1299 cells were selected as the re-search objects,and regular interval short palindromic repeat clusters(CRISPR)technology was used to construct and establish G protein signaling 17(RGS17)KO cells(RGS17 KO group),with H1299 as the control group.The proliferation levels of cells in the control group and RGS17 KO group were analyzed by clone formation experiments and cell counting kit-8(CCK-8)experiments.The migration and invasion abil-ities in the control group and RGS17 KO group were analyzed by wound healing and Transwell.The chan-ges in markers of epithelial and stromal cells in two groups of cells were analyzed by Western blotting.The expression levels of FoxP2,Cyclin D1 and KCIP-1 in two groups were analyzed by fluorescence quantitative polymerase chain reaction(PCR).The intergroup measurement data were analyzed by t-test.Results The expression level of G protein signaling regulator 17 protein in BEAS-2B cells(0.71±0.11)was signifi-cantly lower than that in lung cancer cells(A549,H1299,H446 and H1703)(1.07±0.16,1.96± 0.26,1.40±0.09,1.26±0.13,t=4.965,11.650,12.730,P＜0.05).The colony formation rate of cells in the RGS17 KO group[(50.14±8.31)％]was significantly lower than that in the control group[(82.14±7.08)％,t=7.752,P＜0.05].The absorbance value of RGS17 KO group cells(1.45± 0.13)was significantly lower than that of the control group cells(2.15±0.13,t=1.200,P＜0.05).The scratch healing rate of cells in the RGS17 KO group[(64.71±5.22)％]was significantly lower than that in the control group[(88.43±3.51)％,t=9.980,P＜0.05].The total number of invasive cells in the RGS17 KO group[(77.43±11.53)cells]was significantly less than that in the control group[(128.43± 10.01)cells,t=8.835,P＜0.05].The expression level of E-cadherin in the RGS17 KO group(1.15± 0.15)was significantly higher than that in the control group(0.69±0.09,t=6.904,P＜0.05).The ex-pression level of N-cadherin and[3-catenin in the RGS17 KO group(0.71±0.08,0.91±0.08)was sig-nificantly lower than that in the control group(1.29±0.12,1.53±0.13,t=10.640,10.990,P＜0.05).The expression levels of FoxP2,Cyclin D1 and KCIP-1 in the RGS17 KO group(0.49±0.13,0.50±0.12,0.57±0.08)were significantly lower than those in the control group(1.22±0.10,1.10± 0.10,1.09±0.11,t=11.820,9.993,10.620,P＜0.05).Conclusion The expression level of G protein signaling regulatory factor 17 significantly increases in lung cancer cells,promoting processes such as proliferation and metastasis of lung cancer cells,which may be related to gene expression that activates the cell cycle.
G protein signaling regulatory factor 17Lung cancerProliferationTumor me-tastasis
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