Effect of FK1706 on bladder morphology recovery in rats with neurogenic bladder
Objective To investigate the effect of FK1706 on bladder morphology recovery after end-to-side neurorrhaphy in rats with neurogenic bladder(NB).Methods A total of 40 rats were random-ly divided into the FK1706 and end-to-sidc nerve coaptation group(FK1706+ECG,n=15),end-to-side nerve coaptation group(ECG,n=15),and surgical control group(SCG,n=10).In the FK1706+ECG,the left L6 and S1 ventral root(VR)and dorsal root(DR)were transected,and the distal stump of the L6 VR was sutured to the lateral face of the L4 VR.The ECG was subjected to the same procedure as the FK1706+ECG.In the SCG,the left L6 DR,S1 VR and S1 DR were transected.After the operation,the FK1706+ECG was treated with FK1706(0.32 mg/kg)dissolved in dimethyl sulfoxide(DMSO)for 8 weeks,and the ECG and SCG were treated with the same volume of DMSO.At 4 th month after operation,the bladder was removed,the wet weight of the bladder was measured and the thickness of the bladder wall was measured.Thereafter,the bladder specimens were placed in 10％formalin and stored in the refrigera-tor at 4 ℃.The bladder specimens were made into paraffin sections,and then stained with hematoxylin and eosin(HE)and VG,observed and photographed under the microscope.The area of bladder fibrosis in the three groups rats was calculated and compared.T-test was used for comparison between two groups.Results Bladder wet weight and bladder wall thickness were greater in the FK1706+ECG[(0.291± 0.023)g and(1.613±0.051)mm]than in the SCG[(0.122±0.017)g and(1.169±0.035)mm,t=19.463,23.537,P＜0.01],but significantly lower than in the ECG[(0.415±0.032)g and(2.005± 0.075)mm,t=11.345,15.583,P＜0.01].HE staining showed that the bladder morphology in the SCG was normal,the epithelium was flat,the muscle bundles were arranged neatly,and the connective tissue between the muscle bundles was tight.The detrusor in the FK1706+ECG and ECG was irregular and ede-ma,but the detrusor in the FK1706+ECG was less irregular and edema than that in the ECG.According to VG staining,the bladder fibrosis areas in FK1706+ECG and ECG[(16.99±2.45)％vs.(19.99± 2.55)％]were significantly larger than those in SCG[(11.10±1.38)％,t=6.796,9.928,P＜0.01],but the degree of bladder fibrosis in FK1706+ECG was less than that in ECG(t=3.059,P＜0.01).Conclusion FK1706 promotes bladder morphology recovery after end-to-side neurorrhaphy in rats with neurogenic bladder.
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