Effect of collagen type X alpha 1 chain on progression of prostate cancer through the focal adhesion kinase pathway
Objective To explore the effect of collagen type X alpha 1 chain(COL10A1)on the progression of prostate cancer and its action mechanism.Methods Differential expression of gene COL10A1 in prostate cancer and paracancerous tissues was compared using the TCGA database.The hu-man prostate cancer cell lines 22RV1 and DU145 were divided into control group and experimental group,and the vector and COL10A1 plasmid were transfected.After 2 days,the proliferation ability of cells was verified by 5-Ethynyl-2'-deoxyuridine(EdU)staining experiment,plate cloning formation experiment and cell counting kit-8(CCK-8)method,and the migration ability of cells was verified by Transwell experi-ment,scratch healing experiment and Western blotting.The targeting relationship between COL10A1 and p-DDR2/focal adhesion kinase(FAK)axis was explored by Western blotting.The comparison between the two experimental groups was carried out using the independent sample t-test,and the comparison between the multi-experimental groups was analyzed by analysis of variance.Results The proportion of EdU-stained cells[22RV1:(14.760±2.255)％vs.(53.850±4.463)％,t=7.816,P＜0.05;DU145:(20.560±3.198)％vs.(65.470±2.857)％,t=10.470,P＜0.05],absorbance(22RV1:2.076±0.090 vs.2.538±0.075,F=27.820,P＜0.05;DU145:2.194±0.078 vs.2.856±0.087,F=190.700,P＜0.05),number of colony formation(22RV1:48.330±5.364 vs.162.700±13.120,t=8.067,P＜0.05;DU145:132.000±12.100 vs.297.300±17.320,t=7.825,P＜0.05),scratch healing rate[22RV1:(21.330±1.202)％vs.(31.330±1.453)％,t=5.303,P＜0.05;DU145:(25.330±0.882)％vs.(48.670±2.906)％,t=7.683,P＜0.05],and number of migrated cells(22RV1:55.670±4.910 vs.154.000±6.557,t=12.000,P＜0.05;DU145:28.330±5.608 vs.90.330±11.050,t=5.003,P＜0.05)were lower in the control group than in the experimental group.COL10A1 could significantly increase the expression of p-DDR2 protein(22RV1:0.621±0.010 vs.0.690±0.017,t=3.417,P＜0.05;DU145:0.557±0.010 vs.0.734±0.010,t=12.580,P＜0.05)and increase the expression of FAK protein(22RV1:0.487±0.008 vs.0.608±0.005,t=13.530,P＜0.05;DU145:0.441±0.010 vs.0.511±0.011,t=4.658,P＜0.05).Conclusion COL10A1 can promote prostate cancer proliferation and migration through the DDR2/FAK axis.
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