Application of mitochondria targeting probes with aggregation-induced emission properties in the diagnosis and treatment of bone tumors
Objective To investigate the aggregation-induced luminescence(AIE)properties,organelle targeting,single photon imaging and photodynamic therapy(PDT)effects of MeTTPy.Methods Human osteosarcoma cell line MG63 was used as a cell model to explore the biocompatibility of MeTTPy at a concentration of 1 μmol/L.Commercial mitochondrial dyes were used as a comparison object to explore the targeting region and targeting ability of MeTTPy at the subcellular level,and to discuss reactive oxygen species.The killing rate of different concentrations of MeTTPy on tumor cells was investigated by 28 mw/cm2 white light source irradiation,t test was used to determine the significance between the treat-ment group and the control group,and the potential photodynamic therapeutic effect of MeTTPy on MG63 tumor cells was evaluated by live and dead cell staining.Results MeTTPy had good biocompatibility,and its targeting performance to mitochondria had 90％co-staining rate with commercial mitochondrial dyes.MeTTPy was highly specific to mitochondria in living cells.It had strong ability to produce reactive oxygen species(ROS)in situ.Cytotoxicity test showed that compared with the control group,MG63 cell survival rate was not affected when MeTTPy concentration was 2.5 µmol/L(t=1.862,P＞0.05),and the differ-ence was not statistically significant,indicating good biocompatibility.Under the irradiation of low power white light source(28 mw/cm2),MG63 cells could be killed in a large proportion(killing rate＞92％)at a low concentration(2.5 μmol/L),indicating that MeTTPy had a good phototherapy effect on tumor cells.Conclusion MeTTPy has good aggregation-induced luminescence performance,has high targeting to mito-chondria without the need of carriers such as wrapping,and has a high efficiency ROS generation ability,which can provide efficient fluorescence image-guided photodynamic therapy effect.
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