Aggregation-induced emission molecules with highly efficient fluorescence emission in the NIR-Ⅱ for photothermal diagnosis and therapy of osteosarcoma
Objective To explore the photothermal and fluorescence imaging properties of nanop-articles(2TT-oC6B NPs)constructed by novel fluorescent molecules(AIEgens)with aggregation-induced emission(AIE)properties,as well as their killing effect on osteosarcoma and in vivo tumor-targeting abili-ty.Methods First,the in vitro photothermal properties were examined using 808 nm lasers with different powers(0.5,1.0 and 1.5 W/cm2)and different concentrations(0,25,50,100 and 200 μmol/L)of 2TT-oC6B NPs phosphate buffer saline(PBS)solution,and then 143B cells were used as the model cells to explore its biocompatibility and photothermal killing effect,and finally K7M2 cells were used to inoculate the subcutaneous area of BALB/c mice to construct mice tumor models,and the in vivo tumor-targeting a-bility was examined by injecting 2TT-oC6B NPs(5 mg/kg)through the tail vein.Thet test was used to de-tcrmine the significance between experimental and control groups.Results Compared with the control group,2TT-oC6B NPs could reach a maximum temperature of about 70℃ and had Near-infrared-Ⅱ(NIR-Ⅱ)emission imaging ability under 808 nm laser irradiation.CCK-8 experiments showed that com-pared with the control group,the survival rate of 143B cells at 100 μmol/L was not affected(t=1.734,P＞0.05),demonstrating its good biocompatibility.The survival rate of 143 B cells was significantly decreased after 808 nm laser irradiation(t=15.01,P＜0.05),demonstrating its excellent photothermal kill-ing effect.Meanwhile,the live/dead cell staining showed this result more directly.In vivo tumor imaging in mice demonstrated that it could be enriched at the tumor site within 30 min and retained in the tumor for a long time.Conclusion 2TT-oC6B NPs have excellent photothermal conversion performance and fluores-cence imaging capability.It has good biocompatibility,while it shows efficient photothermal killing effect when under 808 nm laser irradiation.At the same time,its ability to rapidly target tumors and long-term in-tratumoral enrichment suggests that it may serve as a good adjuvant treatment modality for surgical resection of bone tumors,making it a high-performing integrated probe for the diagnosis and treatment of osteosarcoma.
NETL
NSTL
万方数据
