Expression of long non-coding RNA POU3F3 in melanoma and its relationship with metastasis
Objective To explore the expression changes of long non-coding RNA(lncRNA)POU3F3 in melanoma and the relationship between lncRNA POU3F3 and melanoma metastasis.Methods Clinical samples from 47 patients admitted to our hospital from January 2022 to May 2023 who underwent surgical treatment were selected as the research subjects.The expression levels of lncRNA POU3F3 in mel-anoma and adjacent tissues was analyzed by fluorescence quantitative polymerase chain reaction(PCR).The expression of focal adhesion kinase(FAK),β-catenin,N-cadherin,and zinc finger transcription factor Snail protein was analyzed by immunohistochemistry in tumor and adjacent tissues.The relationship be-tween lncRNA POU3F3 and transfer related proteins(FAK,β-catenin,N-cadherin,and zinc finger tran-scription factor Snail protein)was analyzed.The effects of lncRNA POU3F3 knockdown on cell migration and invasion was analyzed by Transwell.The comparison of inter group measurement data was done by the t-test.Pearson analysis was used for correlation analysis.Results The expression level of lncRNA POU3F3 in adjacent cancerous tissues(1.23±0.18)was significantly lower than that in melanoma tissues(2.31±0.19,t=27.890,P＜0.05).The expression level of FAK and β-catenin(58.98±11.11,73.38±12.26)was significantly lower than that of melanoma tissue(96.28±11.74,129.60±14.61,t=16.660,20.200,P＜0.05).The expression levels of N-Cadherin and Snail in adjacent cancerous tis-sues(45.83±13.49,55.47±7.35)were significantly lower than those in melanoma tissues(82.17± 21.99,93.47±11.70,t=8.846,18.860,P＜0.05).The expression level of lncRNA POU3F3 and its correlation with the expression levels of FAK,β-catenin,N-Cadherin,and Snail were positively correlated(r=0.587,0.671,0.814,0.344,P＜0.05).The number of migrative and invasive cells with lncRNA POU3F3 knockdown[(45.75±9.19),(36.13±8.76)cells]was significantly less than that in the con-trol group[(92.50±5.04),(66.13±9.51)cells,t=12.610,6.564,P＜0.05].Conclusion ln-cRNA POU3F3 is significantly upregulated in melanoma tissue and is positively correlated with metastasis related proteins FAK,β-catenin,N-Cadherin,and Snail in tumor tissues.Knocking down lncRNA POU3F3 can significantly inhibit the metastatic characteristics of melanoma cells.
Long non-coding RNAMelanomaMetastasisFocal adhesion kinase
NETL
NSTL
万方数据
