Expression and clinical significance of proline glutamate leucine enriched protein 1 in thyroid cancer tissue
Objective To investigate the changes of proline glutamic acid and leucine rich protein 1(PELP1)in thyroid cancer tissue and correlation between PELP1 expression level and tumor proliferation and metastasis.Methods A total of 45 clinical samples of thyroid cancer treated with surgery in the First Affiliated Hospital of Xinxiang Medical College from August 2018 to August 2023 were selected as the re-search subjects,and corresponding adjacent cancerous tissues were used as controls.The expression level of PELP1 protein in adjacent cancerous tissues and thyroid cancer tissues was analyzed by Western blot-ting.Simultaneously the expression levels of cell proliferation marker(PCNA),migrationmarkers(MMP-1,MMP-13),and epithelial mesenchymal transition markers(N-cadherin and vimentin)were de-tecvted.The ability of proliferation,migration,and invasion in control cells and PELP1 KD cells was ana-lyzed using CCK-8 assay,scratch assay,and Transwell assay.The intergroup measurement data were com-pared by thet-test.Results The expression level of PELP1 protein in adjacent tissues(0.93±0.11)was significantly lower than that in thyroid cancer tissues(2.02±0.28,t=24.370,P＜0.05).The expres-sion level of PCNA protein in adjacent tissues(1.01±0.29)was significantly lower than that in thyroid cancer tissues(2.48±0.12,t=19.070,P＜0.05).The expression level of MMP-13 protein in adjacent tissues(0.71±0.10)was significantly lower than that in thyroid cancer tissues(1.53±0.19,t=24.910,P＜0.05).The protein expression levels of N-cadherin and vimentin in adjacent tissues(1.47±0.23 and 1.28±0.16)were significantly lower than those in thyroid cancer tissues(2.38±0.18 and 2.35±0.17,t=20.790,30.130,P＜0.05).The expression level of PELP1 protein was positively correlated with the expression levels of PCNA,MMP-13,N-cadherin,and vimentin proteins(r=0.142,0.251,0.316,0.482,P＜0.05).The absorbance value of the control group cells(2.04±0.14)was significantly higher than that of the PELP1 KD group cells(1.51±0.10,t=20.790,P＜0.05).The migration ability of the control group cells[(85.46±4.85)％]was significantly higher than that of the PELP1 KD group cells[(68.84±8.69)％,t=4.723,P＜0.05].The number of invasive cells in the control group(142.75± 14.56)was significantly greater than that in the PELP1 KD group(78.25±12.27,t=9.583,P＜0.05).Conclusion The expression level of PELP1 protein significantly increases in thyroid cancer tissue,and downregulating PELP1 can suppress the ability of proliferation,migration,and invasion in 山yroid cancer cells.
Proline glutamate leucine enriched protein 1Thyroid cancerMigrationInva-sionProliferation
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