Expression of geminin DNA replication inhibitor gene in pancreatic cancer and clinical prognostic significance
Objective To investigate the clinical significance and prognostic value of different ex-pression levels of geminin DNA replication inhibitor(GMNN)gene in pancreatic cancer patients.Methods Transcriptome data and clinical information of pancreatic cancer were retrieved from The Cancer Genome Atlas(TCGA)database.Bioinformatics methods were employed to analyze the correlation between the ex-pression differences of GMNN genes in pancreatic cancer tissues and their clinicopathological characteris-tics.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment ana-lyses were performed using R software.A Cox proportional regression risk model was constructed to analyze the factors influencing the survival prognosis of pancreatic cancer patients.Additionally,the R package single-sample gene set enrichment analysis(ssGSEA)method was utilized to explore the correlation be-tween the expression level of key genes in pancreatic cancer and immune cell infiltration.The differences between two or more groups were analyzed using the Wilcoxon rank test or the x2 test,respectively.Results GMNN gene mRNA levels varied among pancreatic cancer patients with different pathological grades,and there was a significant correlation between high GMNN expression and pancreatic cancer pathological grade,history of diabetes mellitus,and overall survival(OS)of the patients(P＜0.05).The enrichment analysis of the GO and KEGG pathways revealed that the relevant differential genes mainly affected the reg-ulation of cellular chemical synaptic transmission,cell membrane potential regulation and other signalling pathways.Survival analysis revealed that OS was significantly longer in the low GMNN expression level group than in the high expression group[2.901(1.810,3.518)vs.1.416(1.153,1.717),hazard ratio(HR)=2.229,95％confidence interval(CI):1.451-3.425,P＜0.01],and multifactorial Cox regres-sion analysis showed that tumour residual status and GMNN expression level[HR=2.075(1.286-3.348),P＜0.01]were independent risk factors for OS in pancreatic cancer patients.Immune infiltration analysis revealed a robust correlation between GMNN expression and the infiltration levels of various immune cells,including antigen-presenting cells and dendritic cells.Conclusion High GMNN expression is associated with worse OS in pancreatic cancer patients,serving as an independent risk factor influencing OS.Addi-tionally,GMNN expression exhibits a significant correlation with immune cell infiltration.
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