The role of progesterone immunomodulatory binding factor 1 in endometrial receptivity
Objective To investigate the effect of progesterone immunomodulatory binding factor 1(PIBF1)on endometrial receptivity.Methods A total of 30 SD rats(aged 2 months)were randomly di-vided into sham surgery group,model group,and PIBF1 group by a random number table method.There were 10 rats in each group.The embryo implantation dysfunction models were established rats in the model group and PIBF1 group during the implantation window period.The embryo implantation models in the sham surgery group were establish during the implantation window period.At 30th day before the establish-ment of the models in the PIBF1 group,the adeno-associated viruses overexpressing PIBF1 were injected through the tail vein,and the sham surgery group and model group were injected with the same volume of adeno-associated viruses.The conditions of rats in three groups were observed during the implantation win-dow period.The levels of estradiol(E2)and progesterone(P)in three groups were analyzed by enzyme linked immunosorbent assay.The number of implanted fetal vesicles in three groups was analyzed.The pathological changes in the endometrium of three groups were detected by hematoxylin and eosin(HE)stai-ning.The number of microvessels in the endometrium was analyzed by immunohistochemical analysis.The expression levels of PIBF1 and p-STAT3 in the endometrium of three groups were analyzed by Western blot-ting.The comparison of inter group econometric data was conducted using one-way analysis of variance.Results The serum estradiol levels in the PIBF1 group[(35.33±3.42)pg/ml]were significantly higher than those in the model group[(19.55±2.84)pg/ml,t=11.230,P＜0.05].The serum progesterone levels in the PIBF1 group[(0.58±0.06)pg/ml]were significantly higher than those in the model group[(0.38±0.06)pg/ml,t=7.329,P＜0.05].The number of embryo implantations in the PIBF1 group(9.70±1.49)was significantly greater than that in the model group[(5.30±1.49),t=6.584,P＜0.05].The model group had a disordered arrangement of endometrium,dilation of uterine cavity,and se-vere infiltration of inflammatory cells.The PIBF1 group had relatively abundant endometrial blood vessels and significantly reduced inflammatory cell infiltration.The number of microvessels in the endometrium of rats in the PIBF1 group(11.20±1.69)was significantly greater than that in the model group(6.60± 1.17,t=7.097,P＜0.05).The expression level of PIBF1 protein in the endometrium of rats in the PIBF1 group(1.46±0.16)was significantly higher than that of rats in the model group(0.45±0.07,t=18.600,P＜0.05).The expression level of p-STAT3 protein in the endometrium of rats in the PIBF1 group(0.76±0.08)was significantly higher than that of rats in the model group(0.30±0.04)(t=15.880,P＜0.05).Conclusion Overexpression of PIBF1 significantly improved the receptivity of the endometrium during the implantation window period,mainly through the activation of the STAT3 signaling pathway.
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