Molecular mechanism of microRNA-424 promoting invasion and proliferation of human hepatocel-lular carcinoma cells by inhibiting cell division cycle 37-like protein 1
Objective To explore the molecular mechanism by which microRNA-424(miR-424)regulates the invasion and proliferation of human liver carcinoma cells through cell division cycle 37-like protein 1(CDC37L1).Methods Tumor tissues and adjacent tissues were collected from 20 patients with liver carcinoma at Zhengxing Hospital during a specific time period.The expression levels of the target genes were detected using fluorescent quantitative polymerase chain reaction(PCR).Human liver carcino-ma cell line HepG2 cells were transfected with miR control,miR-424 mimics,and miR-424 inhibitors.The migration and proliferation abilities of the three groups of cells were analyzed using Transwell assay,scratch assay,and cell counting kit-8(CCK-8).Bioinformatics and dual-luciferase reporter gene experiments were used to analyze the target genes of miR-424.T-test was used for comparison between the two groups,and One-way ANOVA was used for comparison between multiple groups,with P＜0.05 indicating statistically significant difference.Results The expression level of miR-424 in adjacent tissues of patients(0.95± 0.15)was significantly lower than that in tumor tissues(2.24±0.32,P＜0.01).The absorbance value of cells in the miR control group(day 2:0.67±0.12;day 3:0.92±0.11)was lower than that in the miR-424 mimic group(day 2:0.89±0.15,P＜0.01;day 3:1.21±0.12,P＜0.01),and higher than that in the miR-424 inhibitor group(day 2:0.48±0.13,P＜0.01;day 3:0.73±0.09,P＜0.01).The distance between the two sides of the scratch in the miR control group[day 2:(8.72±0.71)mm;day 3:(6.33±0.52)mm]was significantly higher than that in the miR-424 mimic group[day 2:(5.21± 0.68)mm,P＜0.05;day 3:(4.05±0.33),P＜0.01],and lower than that in the miR-424 inhibitor group[day 2:(13.12±2.04)mm,P＜0.01;day 3:(8.12±0.45)mm,P＜0.01].The number of cells passing through the micro-porous membrane per unit area in the miR control group[(39.23± 8.11)cells]was significantly less than in the miR-424 mimic group[(82.50±5.28)cells,P＜0.01],and greater than in the miR-424 inhibitor group[(32.25±4.94)cells,P＜0.05].CDC37L1 is a target gene of miR-424.The expression level of CDC37L1 in HepG2 cells transfected with miR control(0.97± 0.12)was significantly higher than that in the miR-424 mimic group(0.62±0.13,P＜0.01),and lower than that in the miR-424 inhibitor group(2.74±0.32,P＜0.01).Moreover,the expression level of CDC37L1 in adjacent cancer tissues(1.73±0.25)was significantly higher than that in tumor tissues(1.01±0.13,P＜0.01).There was a significantly positive correlation between the expression levels of miR-424 and CDC37L1(r=-0.648,P＜0.01).Conclusion The expression of miR-424 is significant-ly upregulated in human liver carcinoma cells,leading to the downregulation of CDC37L1 expression and subsequently promoting tumor cell proliferation,migration,and invasive abilities.
MicroRNA-424Cell division cycle 37-like protein 1Hepatocellular carcinomaCell migration,Invasion
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