Expression of phosphoserine transaminase 1 in esophageal cancer tissue and its effects on cell prolif-eration and drug resistance
Objective To investigate the expression of phosphoserine aminotransferase 1 in esoph-ageal cancer tissue and its effects on cell proliferation and drug resistance.Methods Totally,95 cases of esophageal cancer clinical specimens and adjacent tissues were collected as the research objects.The ex-pression level of phosphoserine aminotransferase 1 in adjacent tissues and esophageal cancer tissues was an-alyzed by Western blotting.Human esophageal cancer cell line Ec-9706 and cisplatin-resistant Ec-9706 were randomly divided into control short hairpin RNA(shRNA)group and PSAT1 shRNA group,shRNA control/drug resistant group and PSAT1 shRNA/drug resistant group.The effect of phosphoserine amin-otransferase 1 on the proliferation ability of cells in vitro was analyzed by in vivo and in vitro cell prolifera-tion experiments.The apoptosis level of cells was analyzed by flow cytometry.The expression levels of phosphorylated phosphatidylinositol 3 kinase(PI3K),phosphorylated protein kinase B(Akt),drug resist-ance-related protein P-gp and spliced cysteinyl aspartate-specific protease(Caspase)-3 were analyzed by Western blotting.The t test was used for comparison of quantitative data.Results The expression level of phosphoserine aminotransferase 1 in adjacent tissues(0.91±0.20)was significantly lower than that in e-sophageal cancer tissues(1.76±0.26,t=25.230,P＜0.05).The cell inhibition rate of control shRNA group[(7.14±2.06)％]was significantly lower than that of PSAT1 shRNA group[(54.79±11.97)％,t=9.610,P＜0.05].The tumor volume and weight of nude mice in control shRNA group[(1 289.19± 131.71)cm3,(5.36±0.67)g]were greater than those in PSAT1 shRNA group[(705.66±65.88)cm3,(3.27±0.26)g,t=9.706,7.110,P＜0.05].The inhibition rate of shRNA control/drug resistant group[(21.80±3.39)％]was significantly lower than that of PSAT1 shRNA/drug resistant group[(41.12± 3.59)％,t=9.581,P＜0.05].The tumor volume and weight of nude mice in shRNA control/drug resist-ant group[(799.52±61.71)cm3,(3.68±0.51)g]were greater than those in PSAT1 shRNA/drug re-sistant group[(558.62±44.97)cm3,(2.07±0.27)g,t=7.728,6.932,P＜0.05].The inhibition rate of control shRNA group[(3.78±0.81)％]was significantly lower than that of PSAT1 shRNA group[(26.73±4.64)％,t=11.940,P＜0.05].The inhibition rate of shRNA control/drug resistant group[(16.75±4.95)％]was significantly lower than that of PSAT1 shRNA/drug resistant group[(35.04± 4.05)％,t=6.998,P＜0.05].The protein expression levels of phosphorylated PI3K,phosphorylated Akt and drug resistance-related protein P-gp in control shRNA group(0.91±0.10,1.26±0.18,1.19± 0.12)were significantly higher than those in PSAT1 shRNA group(0.61±0.11,0.91±0.06,0.74± 0.07,t=5.024,4.487,7.693,P＜0.05).Conclusion The expression level of phospho-serine amin-otransferase 1 in esophageal cancer tissues is significantly up-regulated,and it is involved in the prolifera-tion and drug resistance of esophageal cancer cells.
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