Expression of microRNA-148a-3p and solute carrier family 7 member 11 in lung cancer tissues and their effects on ferroptosis of tumor cells
Objective To observe the expression of microRNA(miR)-148a-3p and solute carrier family 7 member 11(SLC7A11)in lung cancer tissues and their effects on ferroptosis of tumor cells.Methods Clinical samples of lung cancer patients admitted to our hospital from June 2020 to June 2023 were selected as the research objects,and adjacent tissues were selected as the control objects.The expres-sion levels of miR-148a-3p and SLC7A11 were analyzed by fluorescence quantitative polymerase chain reac-tion(PCR)and Western blotting.Human non-small cell lung cancer cell line H1299 was divided into miR-NA control group and miR-148a-3p group,and the cell line was constructed by lentiviral infection.The proliferation ability of the two groups was analyzed by cell counting kit-8(CCK-8)and colony formation ex-periment.The volume and weight of the two groups in nude mice were analyzed by in vitro xenograft.The expression level of ferroptosis-related protein glutathione peroxidase 4 was analyzed by Western blotting.The target gene of miR-148a-3p was analyzed by bioinformatics and dual luciferase reporter gene analysis,and the expression level of the target gene was analyzed.The t test was used for comparison of measurement data between groups.Results The expression level of miR-148a-3p in adjacent tissues(1.02±0.19)was significantly higher than that in lung cancer tissues(0.54±0.19,t=14.790,P＜0.05).The mRNA expression level of SLC7A11 carrier family 7 member 11 in adjacent tissues(0.66±0.13)was significantly lower than that in lung cancer tissues(1.48±0.17,t=27.180,P＜0.05).The absorbance(A)of the miRNA control group(1.98±0.11)was significantly higher than that of the miR-148a-3p group(1.48± 0.19,t=5.674,P＜0.05).The colony formation rate of the miRNA control group[(85.07±4.50)％]was significantly higher than that of the miR-148a-3p group[(53.68±8.89)％,t=7.714,P＜0.05].The tumor volume of the miRNA control group[(888.40±90.22)mm3]was significantly greater than that of the miR-148a-3p group[(609.78±102.92)mm3,t=6.438,P＜0.05].The tumor weight of the miRNA con-trol group[(5.34±0.75)g]was significantly greater than that of the miR-148a-3p group[(2.28±0.71)g,t=9.379,P＜0.05].SLC7A11 is the target gene of miR-148a-3p.The SLC7A11 of the miRNA control group(0.78±0.07)was significantly higher than that of the miR-148a-3p group(0.48±0.09,t=7.580,P＜0.05).The glutathione peroxidase 4 of the miRNA control group(1.09±0.09)was signifi-cantly higher than that of the miR-148a-3p group(0.39±0.09,t=15.480,P＜0.05).Conclusion The expression of miR-148a-3p is significantly down-regulated in lung cancer tissues,and it regulates fer-roptosis of lung cancer cells through SLC7A11 and affects the growth and development of lung cancer.
MicroRNASolute carrier family 7 member 11Lung cancerFerroptosis
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