LINC01116 interference affects the proliferation,apoptosis,migration and invasion of renal cell carcinoma cells by microRNA-9-5p
Objective To explore the role and possible mechanism of long non-coding RNA(lncRNA)LINC01116 in the development of renal cell carcinoma(RCC).Methods The contents of LINC01116 and microRNA(miR)-9-5p were detected by quantitative real-time polymerase chain reaction(RT-qPCR).Dual luciferase reporter assay verified the binding between LINC01116 and miR-9-5p.The cell counting kit-8(CCK-8)assay,colony formation assay,and flow cytometry were adopted to detect cell prolif-eration,migration,invasion,and appotosis,respectively.The phenotype-related proteins were detected by Western blotting.Results LINC01116 expression in RCC tissues was higher(2.68±0.15 vs.1.02±0.08,P＜0.05),while miR-9-5p expression was lower(0.31±0.04 vs.1.04±0.09,P＜0.05)than in adjacent tissues.LINC01116 targeted miR-9-5p and negatively regulated its expression(P＜0.05).As compared with the control group or si-NC group,the absorbance(A)value(0.69±0.06,0.66±0.08 vs.0.35±0.05),clone formation number(117.37±14.71,115.54±12.42 vs.51.71±3.46),migration number(158.11± 11.92,159.89±11.81 vs.56.56±5.59),invasion number(117.78±7.51,118.33±7.70 vs.35.56± 3.43)and protein levels of proliferation cell nuclear antigen(Ki-67)(0.51±0.09,0.53±0.05 vs.0.17± 0.04),matrix metalloproteinase(MMP)-2(0.73±0.09,0.75±0.04 vs.0.27±0.05)and MMP-9(0.79±0.07,0.77±0.08 vs.0.34±0.05)in the si-LINC01116 group were significantly higher(P＜0.05),while apoptosis rate[(3.07±0.40)％,(3.14±0.46)％vs.(25.47±2.23)％]and cleaved cystei-nyl aspartate-specific protease(cleaved-Caspases-3)protein(0.28±0.04,0.27±0.05 vs.0.62±0.08)in cells were decreased(P＜0.05).As compared with si-LINC01 116+anti-miR-NC group,786-O cells in si-LINC01116+anti-miR-9-5p group showed increased A value(0.33±0.07 vs.0.57±0.07),clone formation number(50.14±3.09 vs.101.97±11.87),migration number(57.89±6.29 vs.126.89±8.02),invasion number(36.11±4.62 vs.96.89±5.73)and Ki-67 level(0.16±0.05 vs.0.35±0.08,P＜0.05),but decreased apoptosis rate[(26.06±2.54)％vs.(6.07±0.71)％]and cleaved-Caspases-3 protein(0.64± 0.06 vs.0.29±0.06)in cells(P＜0.05).Conclusion LINC01116 interference could reduce the prolifer-ation,migration and invasion,but induce apoptosis in RCC cells.
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