Effect of proteasome 26S subunit,non-ATPase 7 on bladder cancer proliferation and migration
Objective This study aims to investigate the effect of PSMD7 expression on the prolif-eration and migration ability of clear cell renal cell carcinoma.Methods The differential expression and prognosis of PSMD7 in clear cell renal cell carcinoma were analyzed using Gepia and Timer databases.Short hairpin RNA(shRNA)was introduced into the Caki-1 cell line to knock down PSMD7 using CRISPR/Cas9 technology,then the experimental grouping was set as Caki-1 control cell line and the exper-imental group of stabilized knockdown of PSMD7(shPSMD7-1,shPSMD7-2).The knockdown efficiency of PSMD7 in Caki-1 cells was determined by Western blotting.The proliferation and migration abilities of the experimental and control groups were assessed by cell counting kit(CCK-8)proliferation assay,plate clo-ning assay,and Transwell assay.Comparisons between the two groups were made using an independent samples t-test.Results PSMD7 expression was significantly upregulated in clear cell renal cell carcino-ma.The expression of PSMD7 was higher in tumor tissues than in normal tissues[5.7(4.13-7.29)vs.3.46(1.74-5.17),P＜0.05].The efficiency of PSMD7 knockdown was determined by Western blotting(1.36±0.05 vs.0.43±0.07,t=10.77,P＜0.05;1.36±0.05 vs.0.31±0.07,t=12.53,P＜0.05).Following PSMD7 knockdown,the proliferative capacity of PSMD7 knockdown cells significantly de-creased,as shown by the CCK-8 results(1.32±0.91 vs.0.67±0.49,t=3.448,P＜0.05;1.32±0.91 vs.0.60±0.35,t=3.222,P＜0.05).Additionally,the colony formation ability of PSMD7 knockdown cells was significantly reduced,as demonstrated by the plate colony formation assay(792.0±24.0 vs.520.0±28.0,t=7.376,P＜0.05;792.0±24.0 vs.518.0±30.0,t=7.132,P＜0.05).The Tran-swell assay revealed a significant decrease in cell migration ability following PSMD7 knockdown(690.0± 14.0 vs.237.5±33.5,t=12.46,P＜0.05;690.0±14.0 vs.176.5±13.5,t=26.4,P＜0.05).Conclusion The study found that PSMD7 was upregulated in clear cell renal cell carcinoma.Knockdown of PSMD7 effectively inhibited the proliferation and migration ability of clear cell renal cell carcinoma.
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