Establishment of an intimal hyperplasia mouse model based on infrarenal abdominal aorta clamping
Objective To set up a stable,efficient infrarenal abdominal aorta intimal hyperplasia mouse model.Methods An existing clamping method was modified and applied in mouse(33 C57BL/6 mice,male,8-12 weeks old)infrarenal abdominal aorta.The 24 mice were randomly divided into 2 groups(28 days after surgery,n=12):control group and clamping group.The rest 9 mice were randomly divided into 3 groups(n=3):control group,0 day and 4 days after surgery.The control group only underwent an-esthesia and the clamping group underwent infrarenal abdominal aorta clamping.The mouse infrarenal ab-dominal aortas were taken after animal sacrifice for hematoxylin and eosin(HE)staining,verhoeff s van Gieson(EVG)staining and immunofluorescence staining of cluster of differentiation 31(CD31)for histolo-gy analysis.The distribution of lesions was observed,the circumference of external,internal elastic lami-nae and the media area were measured and compared.The signal of CD31 immunofluorescence staining was observed.The t-test was used for data analysis.Results All mice survived from surgery and 92％(11/12)of the clamped mice developed intimal hyperplasia lesion 28 days after surgery.The lesion distri-bution analysis showed that most of the lesions were located in the approximal part of the aorta.The circum-ference of the internal elastic lamina in the clamping group was longer than the control group[(1 769.21± 77.61)μmvs.(1 616.16±55.05)μm,t=5.202,P＜0.0001],the circumference of the external elas-tic lamina in the clamping group was longer than the control group[(1 836.75±97.10)μm vs.(1 645.03±80.19)μm,t=5.270,P＜0.0001]and the media area in the clamping group was also lar-ger than the control group[(38 079.89±8 711.61)μm vs.(29 808.88±2 753.53)μm,t=3.140,P＜0.005].The CD31 staining results showed the process of endothelium denudation and re-endothelial-ization.Conclusion The study has established a new mouse which is more stable and efficient for aorta intimal hyperplasia research.
Abdominal aortaIntimal hyperplasiaInjuryAnimal model
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