Effect of high expression of adaptin-ear-binding coat-associated protein 2 on the biological function of hepatocellular carcinoma
Objective To investigate the relationship between the expression of adaptin-ear-bind-ing coat-associated protein 2(NECAP2)in hepatocellular carcinoma(HCC)and the prognosis of HCC pa-tients,and to study the expression of NECAP2 in MHCC-97H and its effect on HCC proliferation and inva-sion.Methods The cancer genome atlas(TCGA)-LIHC were downloaded from TCGA database.A total of 16 HCC samples and their paired adjacent samples were selected and the differential expression levels of normal liver cells and MHCC-97H were detected by real-time fluorescent quantitative polymerase chain re-action(RT-qPCR).MHCC-97H cells were transfected with small interfering RNA to silence NECAP2 ex-pression,and si-NECAP2 group and si-NC group were set up.Cell viability,proliferation,migration,in-vasion,and epidermal growth factor receptor(EGFR)protein expression were determined by cell counting kit(CCK-8),5-ethynyl-2'-deoxyuridine(EdU),Transwell,and Western blotting.Comparison between the two groups was performed by using the t-test.Results Based on the HCC cell group and normal hepa-tocyte group in TCGA database,the expression level of NECAP2 in adjacent tissues was significantly lower than that in HCC tissues(3.747±0.474 vs.4.367±0.593,t=7.102,P＜0.05);The NECAP2 expres-sion in normal liver tissues was significantly lower than that in paired HCC tissues(3.746±0.474 vs.4.263±0.503,t=5.152,P＜0.05).Area under the ROC curve(AUC)value of 0.802 showed that NECAP2 had a good diagnostic value for HCC.The results of the Kaplan-Meier analysis showed that NECAP2 patients in the high expression group had a shorter survival period.The absorbance values,pro-portion of EdU positive cells,number of migrating cells and number of invasive cells[3.201±0.300,(15.06±8.90)％,(103.000±7.550)cells,(112.333±5.508)cells]were significantly reduced in si-NECAP2 group as compared with those in si-NC group[(4.852±0.462),(49.070±10.520)％,(141.000±8.888)cells,(146.333±4.041)cells,t=5.995,4.269,5.664,8.621,P＜0.05].Moreover,EGFR expression was even lower and positively correlated with NECAP2 expression.Conclusion The expression of NECAP2 was significantly upregulated in HCC,and prognosis of the pa-tients with higher NECAP2 expression was worse.The cell experiments proved that downregulation of NECAP2 expression may inhibit the proliferation,migration and invasion of HCC by downregulation of EGFR expression.
Adaptin-ear-binding coat-associated protein 2Hepatocellular carcinomaPrognosisProliferationInvasion
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