肌源性与脂肪源性干细胞治疗尿道括约肌功能障碍型压力性尿失禁大鼠的对比研究
Comparative experimental study on muscle-derived stem cells and adipose-derived stem cells in the treatment of urethral sphincter dysfunction stress urinary incontinence in rats
崔林刚 1孟庆军 1何翔飞 2陈燕 3文建国2
作者信息
- 1. 郑州大学第一附属医院泌尿外科,郑州 450052
- 2. 郑州大学第一附属医院泌尿外科,郑州 450052;河南省尿动力学中心,郑州 450052
- 3. 河南省尿动力学中心,郑州 450052
- 折叠
摘要
目的 探讨肌源性干细胞(MDSCs)和脂肪源性干细胞(ADSCs)分别治疗尿道括约肌功能障碍型压力性尿失禁(ISD)大鼠模型的效果及差异.方法 分离大鼠的MDSCs和ADSCs并鉴定其种类.构建ISD小鼠模型,A组ISD大鼠的膀胱颈部和尿道外括约肌注射MDSCs,为MDSCs组(20例);B组注射ADSCs,为ADSCs组(20例);C组注射D-Hanks培养液作对照组(20例).3组大鼠分别于细胞注射前及第15、30、60天,使用尿动力学检测仪评估其排尿功能.结果 尿动力学检查显示与对照组比较,MDSCs注射治疗和ADSCs注射治疗均都改善了 ISD大鼠的排尿功能,且MDSCs治疗组疗效改善效果更明显.MDSCs组及ADSCs组中的最大尿道压(MUP)(16.95±3.80、12.71±3.30)和漏尿点压力(LPP)(58.49±7.60、45.61±8.80)明显高于对照组(9.37±1.60、28.77±5.10,P＜0.05),且细胞移植后30 d及60 d较细胞移植前明显高,较对照组最大膀胱容量(MCC)(1.86±0.9)比较,MDSCs 组及 ADSCs 组中的 MCC(0.95±0.60、1.30±0.20)明显降低(P＜0.05).结论 MDSCs和ADSCs注射对ISD的治疗有明显的作用,且MDSCs注射效果优于ADSCs.
Abstract
Objective To investigate the effects and differences of muscle-derived stem cells(MD-SCs)and adipose-derived stem cells(ADSCs)in treating urethral sphincter dysfunction stress urinary in-continence(ISD)in rats.Methods MDSCs and ADSCs of rats were isolated and identified.After estab-lishing ray ISD model,the rats injected with MDSCs into bladder neck and external urethral sphincter served as MDSCs group(20 cases),and those injected with ADSCs served as ADSCs group(20 cases).The animals injected with D-Hank's culture solution served as control group(20 cases).The micturition function of three groups was evaluated by urodynamic detector before and 15,30 and 60 days after cell in-jection,respectively.Then the rats were killed.Results Urodynamic examination showed that as com-pared with the control group,both MDSCs injection therapy and ADSCs injection therapy improved the mic-turition function of ISD rats,and the therapeutic effect of MDSCs treatment group was more obvious.The maximum urinary pressure(MUP)in MDSCs group and ADSCs group was(16.95±3.80)and(12.71± 3.30),and the leak point pressure(LPP)was(58.49±7.60)and(45.61±8.80),which were signifi-cantly higher than in the control group(9.37±1.60,28.77±5.10).At 30th and 60th after the transplan-tation,the Maximum bladder capacity(MCC)in MDSCs group and ADSCs group decreased significantly as compared with control group(0.95±0.60 and 1.30±0.20 vs.1.86±0.90,P＜0.05).Conclusion Injection of MDSCs and ADSCs has obvious effects oi the treatment of ISD,and efficacy of MDSCs injec-tion is better than ADSCs injection.
关键词
肌源性干细胞/脂肪源性干细胞/尿道内括约肌障碍/压力性尿失禁/尿动力学评估Key words
Muscle derived stem cells/Adipose tissue-derived stem cells/Intrinsic sphincter deficiency/Stress urinary incontinence/Urodynmic examination引用本文复制引用
出版年
2024
NETL
NSTL
万方数据