Effects of circular RNA special AT rich sequence binding protein 2 on the malignant progression of prostate cancer cells
Objective To investigate the expression changes of circular RNA(circRNA)special AT rich sequence binding protein 2(SATB2)in prostate cancer tissues and its effects on the proliferation,migration and epithelial mesenchymal transition of prostate cancer cells.Methods A total of 123 cases of prostate cancer tissues and corresponding adjacent tissues admitted to our hospital from June 2020 to 2023 were selected as the research objects.The expression level of circRNA SATB2 in prostate cancer and adja-cent tissues was analyzed by fluorescence quantitative polymerase chain reaction(PCR).The circRNA SATB2 knockdown and control stable cell lines were constructed by lentivirus infecting human prostate cancer cell line PC-3.The cell viability of the two groups was analyzed by colony formation experiment and methyl thiazolyl tetrazolium(MIT)experiment.The migration and invasion ability of the two groups was analyzed by scratch experiment and Transwell experiment.The target genes of circRNA SATB2 were ana-lyzed by bioinformatics and double luciferase reporter gene analysis.The expression level of circRNA SATB2 target genes or proteins was analyzed by fluorescence quantitative PCR and Western blotting.The t test was used for comparison of measurement data between groups.Results The expression level of circu-lar RNA SATB2 in adjacent tissues(0.74±0.15)was significantly lower than that in prostate cancer tis-sues(1.57±0.26,t=30.660,P＜0.05).The absorbance value of the cells in the circular RNA control group(2.08±0.11)was significantly higher than that in the circular RNA SATB2 KD group(1.64± 0.09,t=7.289,P＜0.05).The clonal formation rate of cells in the circular RNA control group[(85.40±5.27)％]was significantly higher than that in the circular RNA SATB2KD group[(85.40± 5.27)％,t=10.370,P＜0.05].The clonal formation rate of cells in the circular RNA control group[(85.40±5.27)％]was significantly higher than that in the circular RNA SATB2KD group[(55.48± 4.71)％,t=10.370,P＜0.05].The scratch healing rate in the circular RNA control group[(84.94± 3.67)％]was significantly higher than that in the circular RNA SATB2 KD group[(60.70±12.32)％,t=4.662,P＜0.05].The cell mobility in the circular RNA control group[(66.26±7.96)％]was signif-icantly higher than that in the circular RNA SATB2 KD group[(30.92±5.24)％,t=9.083,P＜0.05].The expression level of miR-760 in control group cells(0.94±0.12)was significantly lower than that of circular RNA SATB2 KD group cells(1.93±0.12,t=14.560,P＜0.05).The expression levels of KIF2A and PHLPP2 in control group cells(0.79±0.08,1.00±0.11)were significantly lower than those in circular RNA SATB2 KD group cells(1.74±0.25,2.02±0.12,t=9.585,14.940,P＜0.05).Conclusion circRNA SATB2 is highly expressed in prostate cancer tissues,and promotes the prolifera-tion,migration and invasion of prostate cancer cells,which may be related to miR-760.
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