Key genes affecting prognosis of prostate cancer based on The Cancer Genome Atlas databases
Objective To explore the key genes affecting the prognosis of patients with prostate cancer based on The Cancer Genome Atlas(TCGA)databases by bioinformatics method.Methods The data of patients with prostate cancer were downloaded from TCGA,and the differentially expressed genes(DEGs)were screened by RStudio software.The DEGs were enriched by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Gnomes(KEGG)by cluster Profile,and screened by Gene Expression Profiling Interactive Analysis 2(GEPIA2)for prognostic genes for prostate cancer.The receiver operating character-istic(ROC)curve was plotted according to the expression level of the genes.Three genes with area under curve(AUC)＞0.7 were divided into high expression group and low expression group according to the op-timal cut-off value,and survival analysis and multivariate regression analysis were performed.The correla-tion of immune cell infiltration was analyzed by timer2.0.Chi-square test,Kaplan-Meier and Cox regres-sion analysis were used for data analysis.Results Totally,358 DEGs were screened.GO and KEGG a-nalysis revealed differences in hormone metabolic proces,peptidase regulator activity,estrogen signaling pathway and other functions and pathways.In total,12 differential genes were screened initially by GE-PIA2.Three genes were selected(AUC＞0.7),the up-regulated genes were progestin,adipoQ receptor 6(PAQR6)and anti-Müllerian hormone(AMH),and the down-regulated gene was amine oxidase copper-containing 1(AOC1).Survival analysis showed that Gleason score,PAQR6,AMH and AOC1 affected o-verall survival(OS)in patients with prostate cancer(x2=7.235,13.513,12.264,13.748,P＜0.01).Multivariate Cox regression analysis showed that Gleason score＞7[hazard ratio(HR):5.76,95％confi-dence interval(CI):1.04-32.07,P＜0.05],high expression of PAQR6(HR:11.75,95％CI:1.42-97.11,P＜0.05)and high expression of AOC1(HR:0.17,95％CI:0.04-0.85,P＜0.05)were inde-pendent predictive factors for OS in patients with prostate cancer.The expression of PAQR6 in prostate cancer was correlated with the expression of CD4+T cells,CD8+T cells and B cells negatively(r=-0.158,-0.200,-0.142,P＜0.01).Conclusion PAQR6 and AOC1 are differentially expressed in prostate cancer,which are independent factors affecting the prognosis of patients with prostate cancer.
NETL
NSTL
万方数据
