Construction of human immune remodeling immunodeficient mouse model
Objective To explore the feasibility of constructing a humanized immune reconstituted mouse model by reinfusing human peripheral blood mononuclear cells(PBMCs)via the tail vein.Methods We recruited 5 blood donors and drew 20 ml venous blood from the median cubital vein.Then we obtained human PBMCs from the peripheral venous blood.NOG mice(obtained from Beijing Vital River Laboratory Animal Technology Co.,Ltd.,China)were divided into 5 groups(Each blood donor corresponds to a group of mice,and each group was divided into three subgroups according to three different injection con-centrations,respectively 1 × 106/mouse,1 × 107/mouse,and 1 × 108/mouse).One week later,blood samples were collected from the orbital plexus of the mice,stained with human CD45 antibody(Biolegend,USA),and the proportion of human CD45+PBMC cells was detected by flow cytometry,followed by t-test analysis.Results The proportion of human CD45+PBMCs in the peripheral blood of NOG mice that re-ceived 1 × 107 cells per mouse reached over 70.0％.In donor group one,the proportion of human CD45+PBMC cells in peripheral blood of mice in the second group was higher than that of mice in the first group[(73.17±1.73)％vs.(40.23±1.71)％,F=483.982,P＜0.01],but not significantly different from that in the third group[(73.17±1.73)％vs.(69.18±2.07)％,F=1.630,P＞0.05].Additionally,the average duration of adverse reactions of mice in the second group was longer than that in the third group[(28±0)d vs.(21±2)d,F=36.750,P＜0.01].These results indicate the successful establishment of a stable humanized immune reconstituted NOG mouse model.Conclusion A stable humanized immune reconstituted mouse model can be effectively established by reinfusing human PBMCs into immunodeficient NOG mice via the caudal vein.
NETL
NSTL
万方数据
