Effect of carbohydrate sulfotransferase 11 on biological behavior in clear cell renal cell carcinoma
Objective To investigate the impact of carbohydrate sulfotransferase 11(CHST11)on the biological functions of cell proliferation,migration,and invasion in clear cell renal cell carcinoma(ccRCC).Methods The differential expression of the CHST11 gene in ccRCC tissues and adjacent tis-sues was explored,and the correlation between CHST1 1 expression levels and ccRCC staging,grading,and prognosis value was analyzed using The Cancer Genome Atlas(TCGA)database.A total of 35 cases of ccRCC and adjacent tissues resected were selected during surgery in the Department of Urology,First Hos-pital of Shanxi Medical University from January 2021 to October 2021,and quantitative real time polymer-ase chain reaction(RT-qPCR),immunohistochemistry,and Western blotting were used to detect the gene and protein expression levels of CHST11 in ccRCC tissues,adjacent tissues,and ccRCC cell lines.The 786O and 769P cell lines with knockdown of CHST11 were constructed using RNA interference technology.The proliferative ability of cells was tested using the cell counting kit-8(CCK-8)assay,and the migration and invasion abilities of cells were measured using the Transwell assay.Data analysis was performed using Graphpad Prism 9.0,Student's t-test was used for comparison between two groups,and one-way analysis of variance was used for comparison between multiple groups,with P＜0.05 defining statistically significant difference.Results The bioinformatics analysis revealed that the mRNA expression level of CHST11 in ccRCC tissue was higher than that in the adjacent normal tissue(4.459±0.060 vs.2.437±0.738,t=15.818,P＜0.01),and it was significantly correlated with patients'prognosis survival rate[hazard ratio(HR)=1.490,P＜0.01].The experimental results indicated that in ccRCC tissue,the mRNA(1.865±1.705 vs.1.000±0.956,t=4.949,P＜0.01)and protein expression levels(1.989±0.353 vs.1.000±0.539,t=4.949,P＜0.01)of CHST11 were higher than in normal tissue.CHST11 was mainly located in the cytoplasm and cell membrane,and knocking down the CHST11 gene could inhibit the proliferation,mi-gration,and invasion ability of ccRCC cells.Conclusion CHST11 plays a tumorigenic role in ccRCC,and silencing CHST11 gene can inhibit the proliferation,migration,and invasion of ccRCC cells.
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