Effects of special AT-rich sequence-binding protein 2 on biological behavior of renal cell carcinoma
Objective To investigate the effects of special AT-rich sequence-binding protein 2(SATB2)on biological behavior of renal cell carcinoma(RCC).Methods Human renal cortex proximal convoluted tubule epithelial cells(HK-2)and RCC cells(786-O and ACHN)were selected.The expres-sion level of SATB2 mRNA in HK-2,786-O and ACHN cells was detected by quantitative real-time poly-merase chain reaction(PCR).Recombinant overexpressed plasmid pcDNA3.1-SATB2 and empty plasmid pcDNA3.1-NC were transfected into 786-O cells,respectively.The cell counting kit-8(CCK-8)was used to detect cell proliferation activity in the two groups.The abilities of migration and invasion were detected by Transwell assay.Western blotting(WB)was used to detect the expression of SATB2,N-cadherin,E-cadherin and Vimentin in the two groups.The inter group comparison was conducted using t-test.Results The expression level of SATB2 mRNA in 786-O cells(0.50±0.06)and ACHN cells(0.57±0.07)was significantly lower than that in HK-2 cells(1.54±0.10,t=23.13,20.23,P＜0.05).The absorbance(A)values of pcDNA3.1-SATB2 cells(0.24±0.02)and pcDNA3.1-NC cells(0.28±0.03)at 24 h were not significantly different(t=2.06,P＞0.05).The A value of pcDNA3.1-SATB2 cells(0.52±0.03,0.80±0.05)at 48 h and 72 h was significantly lower than that of pcDNA3.1-NC cells[(0.69±0.04),(1.11±0.08),t=7.67,8.22,P＜0.05].The number of migrating cells(76.83±6.82)and invading cells(53.33±5.35)of pcDNA3.1-SATB2 cells was significantly less than that of pcDNA3.1-NC cells(135.83±7.96,112.67±5.82;t=13.78,18.38,P＜0.05).The expression lev-els of SATB2(2.01±0.12)and E-cadherin(1.53±0.08)in pcDNA3.1-SATB2 cells were significantly higher than those in pcDNA3.1-NC cells[(0.38±0.03),(0.50±0.05),t=31.82,27.76,P＜0.05].The expression levels of N-cadherin(0.86±0.05)and Vimentin(0.66±0.05)in pcDNA3.1-SATB2 cells were significantly lower than those in pcDNA3.1-NC cells[(1.66±0.08),(1.29±0.05),t=20.16,20.84,P＜0.05].Conclusion SATB2 is low expressed in RCC,and overexpression of SATB2 can inhibit the migration and invasion of tumor cells by regulating epithelial mesenchymal transformation of RCC cells.
Renal cell carcinomaSpecial AT-rich sequence-binding protein 2Epithelial-mesenchymal transitionMigrationInvasion
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