Establishment of renal oxalate stone model in obese mice induced by glyoxalic acid
Objective To establish and verify the model of kidney oxalate stone formation induced by glyoxylic acid in obese mice.Methods A total of 24 6-week-old healthy C57BL/6N male mice without specific pathogen(SPF)were selected and kept in the laboratory of Science Innovation Center of Shanghai 10th People's Hospital after quality inspection by China Institute of Medical Laboratory Animals.Common diet was fed uniformly for 1 week.The mice were divided into two groups by a random number table meth-od:control group(12 mice were fed on ordinary diet);high-fat experimental group(12 mice were fed on high-fat diet for 18 weeks to induce obesity model).The control group of 12 were randomly divided into 6 glyoxylic acid group and 6 PBS group(intraperitoneal injection of phosphate buffer salt solution).Then the high-fat experimental group was randomly divided into obese glyoxylate group 6 and obese group 6.The oxalate content in plasma and kidney of mice in the glyoxylic acid group was determined after intraperitoneal injection of glyoxylic acid solution for 7 consecutive days,and the changes in oxalate content in the 4 groups were compared.A paired t test was used for comparison between the two groups.Measurements conforming to the normal distribution were expressed as mean±standard deviation((x)±s).Results The initial body weight of mice in the 4 groups was from(21.45±2.54)g to(36.42±2.38)g in the control group and(44.91±2.89)g in the high-fat experimental group,with a difference of more than 20％.The average plasma oxalate content in PBS group,obesity group,glyoxylate group and obesity glyoxylate group was compared[PBS group(57.18±39.60)vs.glyoxylate group(281.11±33.54)μmol/L,t=13.62,P＜0.01;PBS group(57.18±39.60)vs.obese group(529.87±241.61)μmol/L,t=5.87,P＜0.01;obesity group(529.87±241.61)vs.obese glyoxylate group(1 156.46±186.83)μmol/L,t=5.41,P＜0.01;glyoxylate group(281.11±33.54)vs.obese glyoxylate group(1 156.46±186.83)μmol/L,t=11.46,P＜0.01].Similarly,the average oxalate content in kidney among all groups was(377.96±148.39)μmol/L in PBS group vs.(805.28±239.49)μmol/L in glyoxylic acid group,t=5.57,P＜0.01 and vs.obese group(1 386.19±310.36)μmol/L,t=6.63,P＜0.01;obesity group(1 386.19±310.36)vs.obese glyoxylate group(3 810.19±477.73)μmol/L,t=11.46,P＜0.01;the glyoxylate group(805.28±239.49)vs.the obese glyoxylate group(3 810.19±477.73)μmol/L,t=14.54,P＜0.01].Conclusion Obesity and intraperitoneal injection of glyoxalic acid can increase the content of ox-alate in plasma and kidney of mice and cause the formation of kidney oxalate stones.
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