Roles of secreted modular calcium binding protein-1 in proliferation and fibrosis of human prostate cells
Objective To explore the differential expression of secreted modular calcium binding protein-1(SMOC1)between hyperplastic prostate and normal prostate,and the effects of SMOC1 on the proliferation,apoptosis and fibrosis of human prostate cells.Methods Based on bioinformatics analysis,public database was used to verify the differential expression of SMOC1 between hyperplastic prostate and normal prostate.Small interfering RNA(siRNA)was used to interfere the expression of SMOC1,and then cell proliferation,cell cycle,cell apoptosis and fibrosis were detected.The inter group comparison was conducted using t-test.Results The relative expression of SMOC1 in hyperplastic prostate was significant-ly higher than normal prostate(6.551±0.201 vs.1.018±0.026,t=19.035,P＜0.001).Knockdown of SMOC1 inhibited cell proliferation(1.087±0.042 vs.1.787±0.051,t=-18.348,P＜0.001;0.942±0.066 vs.1.315±0.063,t=-7.097,P＜0.01)and induced cell cycle arresting at G0/G1 phase(68.310±2.501 vs.61.017±1.897,t=5.411,P＜0.01;67.673±2.357 vs.58.350±2.027,t=5.195,P＜0.01)significantly,while it had no effect on cell apoptosis(3.950±0.628 vs.1.623±0.359,t=12.897,P＞0.05;5.040±0.536 vs.3.100±0.478,t=4.679,P＞0.05).Moreover,knockdown of SMOC1 decreased the expression of cell cycle-and fibrosis-related markers,but it didn't in-fluence the expression of cell apoptosis-related markers.Conclusion Up-regulated SMOC1 in hyperplastic prostate could enhance proliferation and fibrosis of human prostate cells.
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