分泌模块化钙结合蛋白-1在人前列腺细胞的增殖与纤维化中的作用
Roles of secreted modular calcium binding protein-1 in proliferation and fibrosis of human prostate cells
吕源 1叶林朋 1孙浩 1郑航 2杨中华2
作者信息
- 1. 黄梅县人民医院泌尿外科,黄冈 435500
- 2. 武汉大学中南医院泌尿外科,武汉 430071
- 折叠
摘要
目的 探讨分泌模块化钙结合蛋白-1(SMOC1)在人前列腺中的表达差异,以及SMOC1对人前列腺细胞增殖、凋亡和纤维化的影响.方法 基于生物信息学分析,利用公共数据库验证SMOC1在人增生前列腺和正常前列腺中的表达差异;利用小干扰RNA(siRNA)干扰SMOC1的表达后检测细胞增殖、周期、凋亡与纤维化.组间比较采用t检验.结果 增生前列腺组织中SMOC1的相对表达量明显高于正常组(6.551±0.201 比 1.018±0.026,t=19.035,P＜0.001);si-SMOC1 组 BPH-1、WPMY-1 细胞的增殖明显低于对照组(1.087±0.042 比 1.787±0.051,t=-18.348,P＜0.001;0.942±0.066 比 1.315±0.063,t=-7.097,P＜0.01),G0/G1期细胞比例显著高于对照组(68.310±2.501 比 61.017±1.897,t=5.411,P＜0.01;67.673±2.357 比 58.350±2.027,t=5.195,P＜0.01),而早期凋亡和晚期凋亡细胞比例与对照组差异无统计学意义(3.950±0.628 比 1.623±0.359,t=12.897,P＞0.05;5.040±0.536 比 3.100±0.478,t=4.679,P＞0.05).此外,si-SMOC1组细胞周期、纤维化相关标志物蛋白表达水平明显低于对照组,而凋亡相关标志物蛋白表达水平与对照组差异无统计学意义.结论 SMOC1在增生前列腺组织中表达明显上调,而且能够促进前列腺细胞的增殖以及纤维化.
Abstract
Objective To explore the differential expression of secreted modular calcium binding protein-1(SMOC1)between hyperplastic prostate and normal prostate,and the effects of SMOC1 on the proliferation,apoptosis and fibrosis of human prostate cells.Methods Based on bioinformatics analysis,public database was used to verify the differential expression of SMOC1 between hyperplastic prostate and normal prostate.Small interfering RNA(siRNA)was used to interfere the expression of SMOC1,and then cell proliferation,cell cycle,cell apoptosis and fibrosis were detected.The inter group comparison was conducted using t-test.Results The relative expression of SMOC1 in hyperplastic prostate was significant-ly higher than normal prostate(6.551±0.201 vs.1.018±0.026,t=19.035,P＜0.001).Knockdown of SMOC1 inhibited cell proliferation(1.087±0.042 vs.1.787±0.051,t=-18.348,P＜0.001;0.942±0.066 vs.1.315±0.063,t=-7.097,P＜0.01)and induced cell cycle arresting at G0/G1 phase(68.310±2.501 vs.61.017±1.897,t=5.411,P＜0.01;67.673±2.357 vs.58.350±2.027,t=5.195,P＜0.01)significantly,while it had no effect on cell apoptosis(3.950±0.628 vs.1.623±0.359,t=12.897,P＞0.05;5.040±0.536 vs.3.100±0.478,t=4.679,P＞0.05).Moreover,knockdown of SMOC1 decreased the expression of cell cycle-and fibrosis-related markers,but it didn't in-fluence the expression of cell apoptosis-related markers.Conclusion Up-regulated SMOC1 in hyperplastic prostate could enhance proliferation and fibrosis of human prostate cells.
关键词
分泌模块化钙结合蛋白-1/前列腺增生/增殖/纤维化Key words
Secreted modular calcium binding protein-1/Benign prostatic hyperplasia/Prolif-eration/Fibrosis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据