Interfering with circABCC4 targeting microRNA-185-5p to inhibit the development of pancreatic cancer cells
Objective To explore the influence of circABCC4 on the occurrence and development of pancreatic cancer cells and its molecular mechanism.Methods Cancer tissues and adjacent tissues were collected from 39 patients with pancreatic cancer admitted to our hospital from October 2020 to Octo-ber 2023.Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of circABCC4 and miR-185-5p.The dual luciferase reporter experiment was used to de-tect the targeting relationship between circABCC4 and miR-185-5p.Pancreatic cancer cells SW1990 were divided into si-circABCC4 group,si-NC group,miR-185-5p mimic group,miR-NC group,si-circABCC4+miR-185-5p inhibitor group,si-circABCC4+anti-miR-NC group.The tetramethylazolium salt colorimetric method(MTT)was used to detect cell proliferation inhibition rate.The clone formation test was used to de-tect cell clone formation number.The flow cytometry was used to detect cell apoptosis rate.Western blot-ting was used to detect protein expression.Transwell assay was used to test number of migrating and inva-sive cells.This study represented the relevant data using mean±standard deviation((x)±s).The t-test was used for comparing two groups,one-way analysis of variance was used for comparing multiple groups,and LSD-t test was used for pairwise comparisons between groups.P＜0.05 was considered to indicate a signifi-cant difference.Results Compared to adjacent tissues,the expression of circABCC4 was upregulated(3.13±0.29 vs.1.03±0.11,t=42.283,P＜0.05)andtheexpressionofmiR-185-5pwasdownregu-lated(0.45±0.08 vs.1.09±0.17,t=21.273,P＜0.05)in pancreatic cancer tissues.CircABCC4 tar-geted and negatively regulated miR-185-5p expression(P＜0.05).Compared to the si-NC group,SW1990 cells'inhibition rate[(47.13±2.23)％vs.0,F=2 376.899,P＜0.05],apoptosis rate[(18.94±1.08)％vs.(7.33±0.72)％,F=409.972,P＜0.05],andB cell lymphoma/leukemia-2-associated x protein(bax)expression level(0.55±0.04 vs.0.21±0.03,F=310.551,P＜0.05)were elevated in the si-circABCC4 group,while clone formation(67.44±3.44 vs.112.00±5.94,F=296.029,P＜0.05)and B cell lymphoma/leukemia-2(bcl-2)expression level(0.27±0.03 vs.0.67±0.05,F=236.571,P＜0.05)were decreased.Compared to the miR-NC group,SW1990 cells'inhibition rate[(55.52±2.30)％vs.(0.02±0.01)％,F=2 376.899,P＜0.05],apoptosis rate[(22.59±1.45)％vs.(7.11±0.55)％,F=409.972,P＜0.05],and bax expression level(0.65±0.04 vs.0.20±0.02,F=310.551,P＜0.05)were elevated in the miR-185-5p mimic group,while clone formation(54.11±2.23 vs.112.11±6.15,F=296.029,P＜0.05)and bcl-2 expression level(0.17±0.02 vs.0.67±0.07,F=236.571,P＜0.05)were decreased.In comparison with the si-circABCC4+anti-miR-NC group,the si-circABCC4+miR-185-5p inhibitor group showed decreased SW1990 cell inhibition rate[(19.13±0.99)％vs.(47.09±1.73)％,F=2 376.899,P＜0.05],apoptosis rate[(10.25±0.57)％vs.(18.78±1.25)％,F=409.972,P＜0.05],and bax expression level(0.29±0.03 vs.0.57±0.04,F=310.551,P＜0.05),as well as increased clone formation(96.67±4.45 vs.65.78±2.86,F=296.029,P＜0.05),and bcl-2 expression level(0.52±0.04 vs.0.26±0.03,F=236.571,P＜0.05).In comparison to the si-NC group,the si-circABCC4 group showed reduced SW1990 cell mi-gration(97.44±3.65 vs.182.56±10.12,F=576.998,P＜0.05)and invasion(85.56±3.56 vs.150.56±6.50,F=480.065,P＜0.05).Furthermore,compared to the miR-NC group,the miR-185-5p mimic group showed decreased SW1990 cell migration(74.78±3.22 vs.185.56±7.21,F=576.998,P＜0.05)and invasion(71.89±4.18 vs.149.67±6.02,F=480.065,P＜0.05).Lastly,in comparison to the si-circABCC4+anti-miR-NC group,the si-circABCC4+miR-185-5p inhibitor group exhibited increased SW1990 cell migration(158.67±6.15 vs.98.11±2.00,F=576.998,P＜0.05)and invasion(137.89±5.32 vs.85.00±3.40,F=480.065,P＜0.05).Conclusion Interference with circABCC4 inhibits the occurrence and development of pancreatic cancer cells by up-regulating miR-185-5p.
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