Changes of cerebral metabolites in acute liver injury mice with cognitive dysfunction based on 1H-nuclear magnetic resonance
Objective To investigate the changes in the various metabolites at different brain regions in acute liver injury(ALI)mice with cognitive dysfunction(CD).Methods Totally,28 male C57BL/6 mice were randomly assigned to control(8 mice)and ALI(20 mice)groups.Mice that received thioacetamide(TTA)were classified into ALI mice with or without CD-like phenotypes by hierarchical clus-ter analysis of behavior.1 H-nuclear magnetic resonance(1 H-NMR)was used to detect the changes in vari-ous metabolites at different brain regions in ALI mice with CD.The data were analyzed using two independ-ent sample t-test.Results Approximately 55％(11/20)of mice developed CD.Both CD and non-CD groups manifested identical liver injury.Non-CD group spent more time in the novel arm[(111.54±19.32)s vs.(63.96±14.59)s,t=6.28,P＜0.01].Compared to control group,GABA was decreased by 25.08％(0.78/3.11)in the frontal cortex[(3.11±0.34)μmol/g vs.(2.33±0.26)μmol/g,t=5.35,P＜0.05],and Lac was decreased by 32.50％(1.82/5.60)and 38.98％(2.90/7.44)[(5.60±0.35)vs.(3.78±0.75)μmol/g,t=5.98,P＜0.05;(7.44±0.77)vs.(4.54±0.89)μmol/g,t=6.98,P＜0.05],NAA was decreased by 32.12％(1.24/3.86)and 32.74％(1.46/4.46)[(3.86±0.35)vs.(2.62±0.54)μmol/g,t=5.34,P＜0.05;(4.46±0.18)vs.(3.00±0.54)μmol/g,t=6.98,P＜0.05],and Tau was decreased by 34.15％(6.72/19.68)and 26.49％(5.65/21.33)[(19.68±1.69)vs.(12.96±2.43)μmol/g,t=6.32,P＜0.05;(21.33±0.78)vs.(15.68±2.10)μmol/g,t=6.80,P＜0.05]in hippocampus and frontal cortex in the CD group.Conclusion Cerebral neurotransmitter metabolism dysfunction in the frontal cortex and the hippocampus may contribute to ALI-associated CD.
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