Mechanism of microRNA-490-3p targeting high mobility group A2/Wnt5a axis to inhibit prolifera-tion and migration of brain glioma cells
Objective To investigate the effects of microRNA(miRNA,miR)-490-3p on the pro-liferation and migration of brain glioma cells and its molecular mechanism.Methods A total of 70 cases of primary brain glioma specimens and adjacent tissues treated in our hospital from July 2021 to July 2023 were selected as the study objects,and the expression level of miR-490-3p in adjacent tissues and glioma tissues was analyzed by fluorescence quantitative polymerase chain reaction(PCR).Brain glioma cells U251 were resuscitated and divided into control group and miR-490-3p group.Liposomes were transfected into control miRNA and miR-490-3p into U251 cells,respectively.At 48 h after transfection,cell counting kit-8(CCK-8)assay and 5-Ethynyl-2'-deoxyuridine(Edu)staining were used to analyze cell proliferation capacity of the two groups.The in vivo proliferation ability of the two groups of cells was analyzed by alloge-neic tumor formation in vitro.The migration and invasion ability of the two groups of cells was analyzed by scratch test and Transwell test.Bioinformatics and dual luciferase reporter genes were used to analyze the target genes of miR-490-3p,and the expression level of miR-490-3 was analyzed by Western blotting.T test was used to compare measurement data between groups.Results The expression level of miR-490-3p in glioma tissues(0.55±0.11)was significantly lower than that in adjacent tissues(1.18±0.25,t=20.050,P<0.05).CCK-8 absorbance value,EdU staining positive rate and tumor volume[1.93±0.08,(90.66±4.69)%,(1085.27±118.17)mm3]in control group were obviously higher than those in miR490-3p cells[1.47±0.11,(64.12±6.56)%,(685.20±57.28)mm3,t=10.120,10.410,9.634,P<0.05]..The healing rate and number of migrating cells[(80.26±5.51)%,(136.60±10.50)]in control group were obviously higher than those in the miR-490-3p cells[(55.81±6.64)%,(99.50±10.42),t=8.958,7.932,P<0.05].The protein expression levels of HMGA2 and Wnt5a in control group(1.12±0.14,1.21±0.12)were significantly higher than in miR-490-3p group(0.68±0.09,0.75±0.09,t=8.441,10.120,P<0.05).HMGA2 and Wnt5a protein expression levels in brain glioma tissues(1.06±0.14,0.91±0.09)were significantly higher than in paracancer tissue(1.65±0.18,2.18±0.25,t=21.660,41.030,P<0.05).Conclusion MiR-490-3p is low expressed in brain glioma tissues,and it regulates the proliferation and migration of brain glioma cells by targeting and regula-ting the expression level of HMGA2/Wnt5a.
Brain gliomaMicroRNAHigh mobility group A2ProliferationTumor migration