Effects of cerebral ischemia-reperfusion on hippocampal neurons and microglias interleukin-1R1 expression in rats undergoing abdominal surgery
Objective To investigate the effects of cerebral ischemia-reperfusion injury(CIRI)on interleukin-1 R1(IL-1R1)expression in hippocampal neurons and microglia of rats undergoing abdominal surgery.Methods Totally,24 18-month-old SD rats were randomly divided into three groups(n=8 each):sham-operated(Sham)group,abdominal surgery(Surg)group,abdominal surgery+CIRI(Surg+IR)group,which were given general anesthesia without surgical operation,general anesthesia and abdominal surgical treatment,general anesthesia and abdominal surgery and meanwhile common carotid ligation for 10 min,respectively.Then the rats were sacrificed by heart perfusion and the whole brain was stripped 48 h after operation.The hippocampi of the rats were extracted for immunofluorescence staining and confocal observation.The number of IL-1R1 positive neurons and microglias were calculated using Im-age J.One way analysis of variance(ANOVA)was used for comparison between groups.Results The number of IL-1 Ri positive neurons in hippocampal dentate gyrus of Sham group,Surg group and Surg+IR group was 37.50±5.63,42.62±5.73,86.00±6.35,respectively.No significant difference in the num-ber of IL-1R1 positive neurons was observed between Sham group and Surg group(F=3.25,P>0.05),while the number in Surg+IR group was significantly greater than that in Sham group(F=261.36,P<0.05).The number of IL-1R1 positive neurons in Surg+IR group was also significant greater than in Surg group(F=205.83,P<0.05).The number of IL-1R1 positive microglias in hippocampal dentate gyrus of Sham group,Surg group and Surg+IR group was 24.00±3.55,24.87±3.80,103.37±9.35,respec-tively.No significant difference in the number of IL-1R1 positive microglias was observed between Sham group and Surg group(F=0.23,P>0.05),while the number in Surg+IR group was significantly greater than that in Sham group(F=504.12,P<0.05).The number of IL-1R1 positive microglias in Surg+IR group was also significant greater than in Surg group,(F=484.16,P<0.05).Conclusion CIRI upregu-lates IL-1R1 expression levels in hippocampal neurons and microglia of rats undergoing abdominal surgery.