Activation of protein kinase B/murine double minute 2/p53 signaling pathway inhibits proliferation and invasion of non-small cell lung cancer H1975 cells
Objective To observe the effect of regulating the protein kinase B(Akt)/mouse doub-le minute 2(MDM2)/p53 signaling pathway on the malignant biological behavior of non-small cell lung cancer(NSCLC).Methods Logarithmic phase H1975 cell lines(purchased from the U.S.Type Culture Preservation Center)were treated with the Akt/MDM2/p53 signaling pathway activator SC79(4 μg/ml)and inhibitor perifosine(5.0 μmol/L),designated as the SC79 group and perifosine group,respectively.Cells from the untreated H1975 line were used as the control group.The H1975 cell line without any treat-ment was set as blank group.Cell proliferation,apoptosis rate and invasion ability were detected after 24 h intervention.One-way ANOVA was used for comparison among multiple groups and LSD-t test was used for comparison between two groups.Results The absorbance values at 24,48 and 72 h in SC79 group(0.38±0.05,0.63±0.07,0.89±0.12)were higher than those in blank group(0.25±0.04,0.46±0.07,0.62±0.11,t=4.540,3.840,3.709,P＜0.01).The absorbance values in perifosine group at 24,48 and 72 h(0.16±0.04,0.29±0.06,0.40±0.09)were lower than those in blank group(0.25±0.04,0.46±0.07,0.62±0.11,t=3.558,4.123,3.461,P＜0.01).The apoptosis rate[(2.01±0.57)％]in SC79 group was lower than that in blank group[(3.21±0.74)％,t=2.813,P＜0.05].The apoptosis rate in perifosine group[(12.38±3.01)％]was higher than that in blank group[(3.21±0.74)％,t=7.569,P＜0.01].The number of transmembrane cells per field in SC79 group(95.20±15.62)was greater than that in blank group(54.80±9.13,t=4.993,P＜0.05).The number of cells in perifosine group(30.40±8.41)was less than that in blank group(54.80±9.13,t=4.395,P＜0.01).Conclusion Activation of Akt/MDM2/p53 signaling pathway can promote proliferation and invasion,and inhibit apoptosis of NSCLC cells.
Protein kinase BDouble minute 2Non-small cell lung cancerProliferationInvasion
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