Research on modified laparoscopic pancreatic-kidney combined transplantation in porcine model
Objective To explore the feasibility of establishing an modified laparoscopic pancreas-kidney co-transplantation model in living pigs.Methods Totally,16 living pigs were randomly assigned as donors and recipients using a random number table method.In the recipients,bilateral nephrectomy was performed,followed by anastomosis of the donor pancreatic artery and vein to the right renal artery and vein,respectively,with pancreatic juice drainage into the jejunum.The renal artery and vein were anasto-mosed to the internal iliac artery and vein,respectively.The ureter(or renal pelvis)was anastomosed to the left native ureter(or renal pelvis).Intraoperative complications and immediate reperfusion were ob-served.The overall and step-by-step surgery duration and blood loss were described using mean and stand-ard deviation.Results In total,5 out of 8 recipient pigs completed the entire surgical procedure.All recipients underwent successful vascular anastomosis,average surgery duration of(405±9)min and an av-erage blood loss of(122±64)ml.There were two animals experiencing intestinal anastomosis failure,and one experiencing ureteral failure.Among the surviving model pigs postoperatively,three died within 2 h(due to circulatory failure)and two died within 24 h(due to respiratory and circulatory failure).Postmor-tem examinations confirmed the reliability of laparoscopic vascular anastomosis without significant stenosis.Conclusion Performing modified laparoscopic pancreas-kidney combined transplantation in the porcine animal model is feasible in terms of anatomical structure and technical theory.
Pancreas-kidney combined transplantationModel,animalLaparoscopic surgery
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