Impact of long non-coding RNA paternally expressed 10 targeting microRNA-34a on the biological functions of oral squamous cell carcinoma
Objective To investigate the expression of long non-coding RNA PEG10(lncRNA PEG10)in oral squamous cell carcinoma(OSCC)and its effect on OSCC cell proliferation and migration by regulating miR-34a.Methods A total of 53 OSCC tissues and paracancerous tissues were collected as study subjects,and the expression levels of lncRNA PEG10 and miR-34a in the tissues were analyzed by RT-qPCR.SCC-25 cells were divided into si-NC group,si-lncRNA PEG10 group,miR-34a mimics group,and miRNA-NC group by a random grouping method.Cell counting kit(CCK-8)assay,cell scratch assay and Transwell assay were used to detect the proliferation and migration ability of cells in each group.Dual luciferase reporter gene assay was used to verify the targeting relationship between lncRNA PEG10 and miR-34a.Continuous data were compared using independent samples t-test,while categorical data were an-alyzed with Pearson's chi-square test.Results The mean expression of lncRNA PEG10 in OSCC tissues(2.452±0.750)was significantly higher than that in paracancerous tissues(1.293±0.326,t=8.365,P＜0.05).The lncRNA PEG10 expression level in the lncRNA PEG10 group(2.868±0.115)was signif-icantly higher than that in the lncRNA control group(0.973±0.003,t=11.99,P＜0.001).The miR-34a expression level in the mimics group(1.310±0.010)was significantly higher than that in the miRNA control group(0.251±0.020,t=34.16,P＜0.001).High expression of lncRNA PEG10 was correlated with lymph node metastasis(x2=5.577,P＜0.05)and clinical stage(x2=6.606,P＜0.05).The lncRNA PEG10 silencing was associated with the proliferative capacity of SCC-25 cells at 48 h(0.416±0.009)and 72 h(0.555±0.048).The absorbance values of the detection points were signifi-cantly lower than those in the control group(0.561±0.037 and 0.894±0.025,F48 h=590.1,F72h=105.8,P＜0.05).The cell scratch healing rate in the lncRNA PEG10 knockdown group[(157.000±18.457)％]was significantly lower than that in the lncRNA control group[(74.000±14.256)％,t=19.452,P＜0.05].Conclusion lncRNA PEG10 expression was up-regulated in OSCC,while miR-34a expression level was down-regulated.lncRNA PEG10 regulates proliferation and migration of OSCC cells through target binding to miR-34a.
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