Expression and clinical significance of potassium calcium-activated channel subfamily N member 4 in papillary thyroid carcinoma
Objective To investigate the expression of potassium calcium-activated channel sub-family N member 4(KCNN4)in papillary thyroid carcinoma(PTC)and its association with clinical charac-teristics and prognosis.Methods Immunohistochemistry was performed to determine the expression level of KCNN64 in 116 cases of PTC and adjacent normal thyroid tissues collected from the Department of Thy-roid and Breast Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology from January 2021 to June 2021.The clinical characteristics and prognosis were analyzed a-mong patients with different levels of KCNN4 expression.Additionally,the impact of KCNN4 on PTC cell proliferation and migration was assessed using CCK-8 assay and wound healing assay.Unpaired t test was used for comparison between groups.The relationship between KCNN4 and clinicopathological characteris-tics of patients was analyzed by X2 test or Fisher exact test.Survival analysis was performed by Kalpan-Meier method.Results The expression of KCNN4 in PTC was significantly higher than that in adjacent tissues(immunohistochemical score:8.17±2.46 vs.3.26±0.72,t=8.44,P＜0.05).The expression of KCNN4 was significantly correlated with tumor multifocality[low expression group vs.high expression group(the same below),31 vs.50 in single lesion,6 vs.29 in multiple lesions,x2=2.24,P＜0.05],tumor TNM stage(Ⅰ-Ⅱ stage:35 vs.60,Ⅲ-Ⅳ stage:2 vs.19,x2=2.43,P＜0.05)and extrathyroi-dal extension(without extrathyroidal extension:34 vs.59,with extrathyroidal extension:3 vs.20,x2=2.17,P＜0.05),while irrelevantly correlated with age(≤ 55 years old:12 vs.28,＞55 years old:25 vs.51,x2=0.32,P＞0.05),gender(male:5 vs.16,female:32 vs.63,x2=0.88,P＞0.05)and Hashimoto's thyroiditis(without Hashimoto's thyroiditis:23 vs.56,with Hashimoto's thyroiditis:x2=0.94,P＞0.05).The recurrence-free survival rate of patients with high KCNN4 expression was lower than that of patients with low KCNN4 expression(recurrence rate:8.86％vs.0％,HR=2.42,P＜0.05).CCK-8 assay showed the proliferation ability of KTC-1 and BCPAP cells after KCNN4 knockdown was lower than that of control cells[KTC-1 control group vs.short hairpin RNA(shRNA)transfection group,72 h:1.28±0.11 vs.0.47±0.06,t=8.28,P＜0.05;96 h:1.82±0.24 vs.0.61±0.10,t=10.45,P＜0.05;BCPAP control group vs.shRNA transfection group,72 h:1.37±0.19 vs.0.51±0.07,t=6.14,P＜0.05;96h:2.03±0.47 vs.0.84±0.20,t=3.99,P＜0.05].Wound healing assay showed the wound healing ability of KTC-1 and BCPAP cells after KCNN4 knockdown was lower than that of the control group[the percentage of wound healing at 24 h in KTC-1 control group vs.shRNA transfection group:(66.47±7.60)％vs.(33.41±3.76)％,t=6.28,P＜0.05;the percentage of wound healing at 24 h in BCPAP control group vs.shRNA transfection group:(71.25±9.06)％vs.(43.32±5.83)％,t=12.10,P＜0.05].Conclusion These findings suggested that KCNN4 plays a crucial role in the occur-rence and progression of PTC.
Potassium calcium-activated channel subfamily N member 4Papillary thyroid car-cinomaClinical characteristicsPrognosis
NETL
NSTL
万方数据
