Construction of a sustainable blood purification model in New Zealand rabbits
Objective To explore the feasibility and advantages of three kinds of puncture cathe-terization methods to establish a New Zealand rabbit model of sustainable blood purification therapy.Methods A total of 120 New Zealand white rabbits were randomly divided into internal mandibular vein group,common carocid artery group,femoral artery group and control group with 30 rabbits per group.The internal mandibular vein,common carocid artery and femoral artery were respectively punctured and cathe-terized using 22 Gauge Closed Needle Protection Ⅳ Catheter System.No catheterization was performed in the control group.The per time of puncture catheterization,the number of catheterization,postoperative infection and death were recorded.The blood routine,blood glucose,electrolyte and liver function of 4 groups of rabbits were compared.One-way ANOVA,Kruskal-Wallis H test and Chi-squared test were used for statistical analysis.Results The animal model was successfully constructed by all three kinds of puncture catheterization methods.The mean puncture catheterization time in the internal mandibular vein group was shorter than that in the common carotid artery group and the femoral artery group[2.5(2.3,2.6)min vs.6.7(6.4,7.5)min,5.5(5.4,6.1)min,Z=67.110,P＜0.01],the average times were more than those in the common carotid artery group and the femoral artery group(12.0 times vs.7.0 times,8.0 times,Z=58.769,P＜0.01).The postoperative infection rate[3.3％(1/30)vs.6.7％(2/30),6.7％(2/30)]and mortality rate[0.0％(0/30)vs.3.3％(1/30),3.3％(1/30)]were com-pared among the three groups,and there was no statistically significant difference(x2=0.424,1.023,P＞0.05).The postoperative hemameba values in the internal mandibular vein group were lower than those in the common carotid artery group and the femoral artery group[(6.2±1.3)×109 vs.(7.8±1.4)× 109,(7.2±1.1)×109,F=14.571,P＜0.01].Conclusion The New Zealand rabbit model of sustain-able blood purification therapy can be successfully established by the three puncture catheterization meth-ods,and the internal mandibular vein catheterization is preferred.
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