Effect of quercetin on chondrocyte apoptosis induced by interleukin-1 β and underlying mechanism
Objective To explore the effect of quercetin on chondrocyte apoptosis induced by interleukin-1β(IL-1β)and underlying mechanism.Methods The chondrocytes from SD rats were ob-tained by enzyme digestion and divided into 5 groups:control group(without any stimulation);IL-1β group(10 ng/ml IL-1β);low-dose quercetin group(10 ng/ml IL-1β+100 µmol/L quercetin),medium-dose quercetin group(10 ng/ml IL-1β+200 µmol/L quercetin),high-dose quercetin group(10 ng/ml IL-1β+400 μmol/L quercetin).Cell viability was detect by methylthiazol tetrazolium(MTT)assay.Cell apoptosis was detected by Annexin V-propidium iodide(PI)flow cytometry.The expression of B-cell lym-phoma-2(bcl-2),bcl-2-associated X protein(bax),clevead cysteinyl aspartate specific proteinase 3(clev-ead Caspase-3),matrix metalloproteinase-3(MMP-3),MMP-13 and phosphorylation of inhibitor of NF-κB(IκBα)and nuclear factor kappa-B(NF-κB)p65 was detected by Western blotting.Data were analyzed by LSD-t test.Results Cell viability[(0.43±0.03),(0.47±0.02),(0.53±0.04)vs.(0.36±0.03),t=5.421,6.345,6.521,P＜0.01]and the expression of bcl-2[(0.45±0.03),(0.46±0.03),(0.57±0.04)vs.(0.22±0.01),t=6.021,6.239,5.984,P＜0.01]in low-,medium-and high-dose quercetin groups was higher than those in IL-1 β group.Cell early apoptotic rate[(17.73±1.34)％,(11.68±0.90)％,(6.48±0.42)％vs.(21.46±2.28)％,t=5.421,6.345,6.521,P＜0.01],cell late apoptotic rate[(9.46±0.36)％,(6.26±0.52)％,(3.87±0.40)％vs.(15.53±1.25)％,t=5.873,6.126,6.436,P＜0.01],the expression of bax[(0.50±0.04),(0.41±0.03),(0.19±0.01)vs.(1.24±0.09),t=6.325,6.984,6.324,P＜0.01],cleaved Caspase-3[(0.43±0.03),(0.31±0.02),(0.23±0.0)vs.(1.02±0.05),t=5.687,5.214,5.983,P＜0.01],MMP-1[(0.43±0.03),(0.42±0.02),(0.57±0.04)vs.(1.03±0.08),t=6.418,5.647,6.942,P＜0.01],MMP-13[(0.98±0.06),(0.47±0.03),(0.41±0.02)vs.(1.24±0.08),t=6.017,5.283,6.216,P＜0.01],p-IκBα[(0.95±0.06),(0.48±0.03),(0.33±0.02)vs.(1.32±0.11),t=5.487,5.328,5.987,P＜0.05]and p-NF-κB p65[(0.98±0.08),(0.35±0.02),(0.25±0.01)vs.(1.14±0.08),t=6.328,6.457,5.984,P＜0.05]in low-,medium-and high-quercetin groups were lower than those in IL-1β group.Conclusion Quercetin could significantly inhibit IL-1β-induced chon-drocyte apoptosis probably by inhibiting the NF-κB signal pathway.
QuercetinOsteoarthritisChondrocyteApoptosisNuclear factor kappa-B signal pathway
NETL
NSTL
万方数据
