The diagnostic value of serum microRNA-129-5p in osteoarthritis and its relationship with high mobility group protein B1 inflammatory signaling pathway
Objective To investigate the expression level and diagnostic value of serum microRNA-129-5p(miR-129-5p)in osteoarthritis(OA).Methods A total of 126 OA patients who were treated in the Department of Orthopedics of Shanxi Provincial People's Hospital from March 2019 to July 2023 were selected as the observation group,and 105 healthy people who underwent physical examination in the phys-ical examination center during the same period were included as the control group.Real-time fluorescent polymerase chain reaction(RT-qPCR)was used to detect the expression level of serum miR-129-5p.The expression levels of high mobility group protein B1(HMGB1),tumor necrosis factor-α(TNF-α),interleu-kin-1β(IL-1β)and CRP in serum were detected by enzyme-linked immunosorbent assay(ELISA).Pear-son correlation coefficient was used to analyze the correlation between miR-129-5 p,HMGB1 levels and TNF-α,IL-1β,CRP.The correlation between miR-129-5p and HMGB1 was analyzed.Multivariate Logis-tic regression was used to analyze the risk of miR-129-5p on OA.The receiver operating characteristic(ROC)curve was used to determine the diagnostic value of miR-129-5p in OA.Results The serum level of miR-129-5p in the observation group was significantly lower than that in the control group(0.49±0.28 vs.0.99±0.34,t=12.390,P＜0.01).The serum level of HMGB1 in the observation group was significantly higher than that in the control group[(45.68±18.41)vs.(23.20±8.92)ng/ml,t=11.414,P＜0.01].The serum levels of TNF-α,IL-1 β and CRP in the observation group were significantly higher than those in the control group(t=7.331,P＜0.01;t=5.246,P＜0.01;t=8.622,P＜0.01).Serum miR-129-5p level was negatively correlated with HMGB1(r=-0.615,P＜0.01).Multivariate Logistic regression a-nalysis showed that the odds ratio(OR)value of miR-129-5p was 0.019.The ROC curve analysis showed that the cut-off value of serum miR-129-5p was 0.928,which had good sensitivity(95.24％)and specific-ity(59.05％)in predicting OA,and the area under the curve(AUC)was 0.861[95％confidence inter-val:0.815-0.907,P＜0.01].Conclusion The serum level of miR-129-5p is significantly decreased in OA patients,and it is negatively correlated with HMGB1 and inflammatory markers TNF-α,IL-1β and CRP,and has a good diagnostic value for predicting OA.
OsteoarthritisMicroRNAHigh mobility group protein 1Inflammation
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