目的 观察盐酸氢吗啡酮对人胃癌MGC-803细胞的增殖、迁移、侵袭和相关蛋白表达的影响。方法 人胃癌MGC-803细胞随机分为两组(n=6):对照组(C组)和400 µmol/L氢吗啡酮组(H组)。应用细胞计数试剂盒(CCK-8)法检测细胞活力，细胞克隆形成实验检测细胞的增殖能力，Transwell实验检测细胞的侵袭和迁移能力，蛋白质印迹法(Western blot)检测磷酸化磷脂酰肌醇3激酶(p-PI3K)、磷酸化蛋白激酶B(p-Akt)和磷酸化雷帕霉素靶蛋白(p-mTOR)蛋白的表达，组间比较采用方差分析和独立样本t检验。结果 H组的细胞活力明显低于C组[(66。34±7。56)％比(93。76±4。97)％，t=7。421，P＜0。05]，差异有统计学意义。H组胃癌细胞克隆形成能力低于C组[(62。17±4。79)％比(90。83±7。73)％，t=7。720，P＜0。05]，差异有统计学意义。H组细胞迁移数低于 C 组[(101。67±5。43)个比(142。83±6。29)个，t=11。816，P＜0。05]，H组的细胞侵袭数低于C组[(75。83±4。02)个比(94。50±5。96)个，=6。361，P＜0。05]，差异有统计学意义。H组p-PI3K蛋白的表达低于C组(0。24±0。03比0。53±0。04，t=13。915，P＜0。05)，差异有统计学意义。H组p-Akt蛋白的表达低于C组(0。54±0。05比0。64±0。06，t=3。021，P＜0。05)，差异有统计学意义。H组p-mTOR蛋白的表达低于C组(0。17±0。02比0。24±0。03，t=5。081，P＜0。05)，差异有统计学意义。结论 氢吗啡酮抑制MGC-803细胞的增殖、侵袭和迁移能力，其作用可能与抑制PI3K/Akt/mTOR信号通路有关。
Effects of hydromorphone hydrochloride on proliferation,migration and invasion of gastric cancer cells
Objective To observe the effects of hydromorphone hydrochloride on proliferation,inva-sion,migration and related protein expression of human gastric cancer MGC-803 cells.Methods MGC-803 cells were randomly divided into 2 groups(n=6):control group(group C)and 400 µmol/L hydromor-phone group(H group).Cell counting kit-8(CCK-8)assay was used to detect cell viability,cell clonal formation assay was used to detect cell proliferation ability,Transwell assay was used to detect cell invasion and migration ability,Western blotting assay was used to detect the protein expression of phosphorylated phosphatidylinositol 3 kinase(p-PI3K),phosphorylated protein kinase B(p-Akt)and phosphorylated mammalian target of rapamycin(p-mTOR),and variance analysis and independent sample t test were used to analyze the differences between groups.Results Compared with group C,the cell viability[(66.34±7.56)％vs.(93.76±4.97)％,t=7.421,P＜0.05]and the clonal ability[(62.17±4.79)％vs.(90.83±7.73)％,t=7.720,P＜0.05]in H group was significantly decreased.As compared with group C,the number of migrating cells(101.67±5.43 vs.142.83±6.29,t=11.816,P＜0.05)and the num-ber of invasive cells(75.83±4.02 vs.94.50±5.96,t=6.361,P＜0.05)in H group were significantly decreased.As compared with group C,the expression of p-PI3K protein(0.24±0.03 vs.0.53±0.04,t=13.915,P＜0.05),p-Akt protein(0.54±0.05 vs.0.64±0.06,t=3.021,P＜0.05)and p-mTOR protein(0.17±0.02 vs.0.24±0.03,t=5.081,P＜0.05)in H group significantly decreased.Conclusion Hydromorphone inhibited the proliferation,invasion and migration of MGC-803 cells,which may be related to the inhibition of PI3K/Akt/mTOR signaling pathway.
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