Effect of quercitrin on migration and invasion of colon cancer by transforming growth factor beta/samds signal pathway
Objective To explore the effect of quercitrin on migration and invasion of colon cancer by modulating transforming growth factor beta1(TGF-β1)/samds signal pathway.Methods HCT116 co-lon cancer cells were divided into control group,low-,medium-,and high-dose quercetin groups,and trea-ted with 0,5,10,and 20 µmol/L quercetin for 48 h,respectively.Cell viability was measured by methyl thiazolyl tetrazolium(MTT)assay.Cell migration ability was detected by migration test.Cell invasion abil-ity was detected by Transwell test.The expression of matrix metalloproteinase-9(MMP-9),tissue inhibitor of metalloproteinase-1(TIMP-1),Vimentin,E-cadherin,TGF-β1,p-Smad2 and p-Smad3 was detected by Western blotting.Inter group differences were analyzed by F-test.Results Cell viability(0.48±0.01,0.41±0.01,0.34±0.01 vs.0.54±0.02,F=26.354,P＜0.05),the number of migrating cells[(117.62±3.49),(43.36±2.53),(35.56±1.32)vs.(176.48±9.35)F=35.624,P＜0.05],the number of invasive cells[(86.31±3.83),(41.28±2.26),(30.41±1.26)vs.(182.43±9.21),F=36.520,P＜0.05],the expression of MMP-9(0.98±0.05,0.46±0.02,0.32±0.01 vs.0.35±0.01,F=26.546,P＜0.05),Vimentin(0.53±0.03,0.41±0.03,0.20±0.01 vs.0.08±0.00,F=27.649,P＜0.05),TGF-β1(0.82±0.05,0.74±0.04,0.70±0.04 vs.0.35±0.01,F=30.325,P＜0.05),p-Smad2(1.78±0.09,1.56±0.10,0.84±0.05vs.0.41±0.03,F=33.654,P＜0.05)and p-Smad3(1.94±0.12,0.87±0.07,0.56±0.04 vs.0.28±0.01,F=35.674,P＜0.05)protein in low-,medium-,and high-dose quercetin groups were significantly reduced as compared with those in con-trol group.The expression of TIMP-1(0.21±0.01,0.31±0.02,0.46±0.03 vs.0.64±0.04,F=28.413,P＜0.05),E-cadherin(0.09±0.00,0.22±0.01,0.43±0.02 vs.0.89±0.07,F=28.561,P＜0.05)protein in low-,medium-,and high-dose quercetin groups was higher than that in control group.Conclusion Quercitrin could inhibit migration and invasion of HCT116 colon cancer cells probably by suppressing the TGF-β1/smad2/3 signal pathway.
QuercitrinColon cancerMigrationInvasionTransforming growth factor beta1/samds signal pathway
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