Inhibitory effect of programmed cell death ligand-1 inhibitor on neuroglioma cells and its mecha-nism
Objective To investigate the inhibitory effect of programmed cell death ligand-1(PD-L1)inhibitor on malignant biological behavior of neuroglioma cells and its mechanism.Methods Human normal astrocytes HA 1800 and human neuroglioma cells(U87-MG,U251-MG)were selected for the study.Real-time fluorescence quantitative polymerase chain reaction was used to detect the PD-L1 mRNA expression in HA1800,U87-MG,and U251-MG cells.U87-MG cells were divided into two groups:U87-MG cells with the addition of PD-L1 inhibitor(experimental group)and U87-MG cells without PD-L1 inhibitor treatment(control group).The cell proliferation viability in the experimental and control groups was detected using a cell counting kit-8(CCK-8)assay;Transwell was used to detect the migration and in-vasion ability of the experimental group and the control group.The protein expression of PD-L1,phospho-rylation signal transducer and activators of transcription 1(STAT1),and phosphorylation signal transducer and activators of transcription 3(STAT3)in the experimental group and control group was detected by Western blotting.The t-test was used for comparison between groups.Results The expression of PD-L1 mRNA in U87-MG cells(1.73±0.08)and U251-MG cells(1.62±0.06)was significantly higher than that in HA1800 cells(0.49±0.05,t=31.91,37.70,P＜0.05).The absorbance(A)value of the experi-mental group(0.52±0.05,0.82±0.04)at 48 h and 72 h was significantly lower than that of the control group(0.80±0.05,1.32±0.06,t=9.49,17.94,P＜0.05).The number of migrating cells[(73.33±5.35)]and invasive cells[(46.17±5.12)]in the experimental group was significantly less than that in the control group[(135.17±7.52)and(88.50±6.77),t=16.41,12.21,P＜0.05].The protein ex-pression of PD-L1(0.88±0.05)and phosphorylated STAT3(0.44±0.05)in experimental group was sig-nificantly lower than that in control group(1.88±0.06,1.24±0.05,t=30.34,26.73,P＜0.05).The phosphorylated STAT1 in experimental group(1.32±0.08)was significantly higher than that in control group(0.51±0.05,t=20.43,P＜0.05).Conclusion PD-L1 inhibitor can inhibit the proliferation,mi-gration and invasion of programmed cells by up-regulating the phosphorylated STAT1 and down-regulating the phosphorylated STAT3.
Programmed cell death ligand-1 inhibitorNeurogliomaProliferationMigrationInvasion
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