Proteomics and differential expression analysis of prostate cancer tissues
Objective To investigate the proteomics and differential expression of protein in pros-tate cancer tissues.Methods A total of 12 cases of prostate cancer tissue and adjacent tissue treated in our hospital from January 2022 to December 2023 were selected as the study objects.Total protein was ex-tracted from 12 adjacent tissues and prostate cancer tissues using tissue lysate,and tumor samples and nor-mal tissue samples were subjected to two-dimensional electrophoresis using immobilized PH gradient glue with gradient overlap to obtain total protein profiles.Differences between the two groups of profiles were an-alyzed,blot sites with differential expression were selected,and mass spectrometry identification and data-base retrieval were performed after incision.The biological functions of differential proteins were analyzed.The levels of differentially expressed proteins were verified by Western blotting.Measurement data were compared by independent-samples t test.Results In this study,125 differentially expressed protein sites were obtained by two-dimensional electrophoresis.Tandem mass spectrometry was used to obtain peptide fingerprints.A total of 54 significantly expressed proteins were obtained,of which 31 were up-regulated and 23 were down-regulated.The top 5 proteins with increased expression were L-lactate dehydrogenase B,α-enolase,ER protein ERp29,transcription activator 6,and high mobility protein A2.The top 5 proteins with down-regulated expression were protein disulfide isomerase,thrombocytin-1,WW domain REDOX enzyme,ER reticulum protein ERp29 and acetaldehyde dehydrogenase,respectively.These proteins are mainly involved in cellular central carbon metabolism,protein translation,gene transcription regulation,endoplasmic reticulum stress response,extracellular matrix remodeling and other biological processes.The expression levels of L-lactate dehydrogenase B,α-enolase,vimentin,transcriptional activator 6 and high mobility protein A2 in adjacent tissues(0.87±0.09,1.05±0.15,0.75±0.06,0.76±0.09,0.62±0.09)were significantly lower than those in prostate cancer(1.85±0.13,1.71±0.14,1.47±0.12,1.24±0.12,1.40±0.22,t=21.530,11.090,18.930,11.450,11.460,P＜0.05).The expression levels of protein disulfide isomerase,thrombocytin-1,WW domain REDOX enzyme,ER reticulum protein ERp29 and acetaldehyde dehydrogenase protein in adjacent tissues(1.43±0.19,1.14±0.14,1.37±0.14,1.03±0.09,0.91±0.06)were significantly higher than those in prostate cancer(0.42±0.12,0.74±0.11,0.79±0.07,0.69±0.07,0.60±0.13,t=15.430,7.620,12.750,10.280,7.418,P＜0.05).Conclusion The abnormal expression of protein related to carbon metabolism,protein translation and gene transcription regulation in the cell center of prostate cancer may be closely related to the formation and progression of prostate cancer.
Prostate cancerProteomicsDifferentially expressed protein
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