Expression of TP53 in prostate cancer and its correlation with phosphatase and tensin homologue deleted on chromosome ten were evaluated based on bioinformatics analysis
Objective To evaluate the expression levels and correlation of TP53 and phosphatase and tensin homologue deleted on chromosome ten(PTEN)in prostate cancer(PCa)based on bioinformatics analysis.Methods The expression of TP53 gene in prostate cancer and its possible regulatory mechanism were analyzed by mining public databases using bioinformatics methods.A total of 79 cases of PCa diag-nosed and treated in the Department of Urology,the Affiliated Hospital of Xuzhou Medical University from March to August 2022 were collected.The pathological sections were reviewed and the relevant clinical data were sorted out.The protein expression levels of PTEN and TP53 in 79 cases of PCa were detected by im-munohistochemical SP staining,and Pearson correlation analysis was used to analyze their correlation.Chi-square test and Mann-Whitney U test were used for comparison of categorical data between groups.Pearson correlation analysis was used to analyze the correlation between PTEN and TP53 in prostate cancer patients.Results Through TCGA data analysis TP53 expression level was significantly higher in patients with prostate cancer(501 cases vs.52 cases,Z=8 874.5,P＜0.05)in normal people;The relationship between TP53 expression level and clinicopathological parameters of prostate cancer patients showed that(250 cases of lower expression vs.251 cases of high expression):TP53 was not significantly associated with clinical characteristics including age(x2=6.079,P＞0.05),T stage(x2=0.338,P＞0.05),N stage(x2=0.176,P＞0.05),PSA level(x2=0.016,P＞0.05)and Gleason score(x2=3.999,P＞0.05).The nomogram for predicting the overall survival(OS)of PCa was established.The analysis showed that TP53 could not be used as an independent prognostic factor for OS,and PSA value and Gleason score had prognostic effects in PCa.Immunohistochemical staining showed that the positive rate of PTEN protein(intact)was 73％.TP53 protein staining showed wild type mutation pattern in 81％cases,and mutant ex-pression pattern in 19％cases.Pearson analysis showed that PTEN loss had a higher correlation with p53 mutation(r=0.653,P＜0.05).Conclusion TP53 combined with PTEN has a certain predictive effect on the progression of PCa,and has important value in the clinical treatment of prostate cancer.
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