长链非编码RNA浆细胞瘤变异易位1在胃癌中的表达及临床意义
Expression and clinical significance of long noncoding RNA plasmacytoma variant translocation 1 in gastric cancer
陈文亮 1董静逊 2白子豪 3王欢欢 3郝金锦 3陈凛4
作者信息
- 1. 山西医科大学第二医院普通外科,太原 030001
- 2. 山西省肿瘤医院消化微创外科,太原 030013
- 3. 山西医科大学研究生学院,太原 030001
- 4. 北京大学国际医院胃肠外科,北京 102206
- 折叠
摘要
目的 探讨长链非编码RNA PVT1(lncRNAPVT1)在胃癌(GC)组织中的表达及临床意义.方法 首先通过实时荧光定量聚合酶链反应(RT-qPCR)检测196例GC组织中lncRNA PVT1表达,采用x2检验分析其与临床病理特征间关系.利用t检验分析26例GC组织中微小RNA(miR)-30a-3p表达;然后对具有随访资料的182例GC患者,进行生存分析和Cox回归分析.结果 RT-qPCR检测结果显示:癌组织中lncRNA PVT1的表达水平显著高于癌旁组织(0.33±0.15比0.28±0.13,t=3.746,P＜0.01).lncRNA PVT1 表达水平与 GC 肿瘤大小(x2=8.610,P＜0.01)、癌组织分化程度(x2=4.927,P＜0.05)、浸润深度(x2=5.940,P＜0.05)、pTNM 分期(x2=4.675,P＜0.05)及淋巴结转移(x2=4.712,P＜0.05)呈现显著相关性.而GC组织中miR-30a-3p的表达水平显著低于癌旁组织(0.210±0.156 比 0.302±0.177,t=2.378,P＜0.05).生存分析显示:lncRNA PVT1高表达组患者总生存期(OS)明显短于低表达组[风险比(HR)=1.746,95％可信区间(CI):1.154～2.642,P＜0.01].结论 lncRNA PVT1在GC组织中存在表达上调,miR-30a-3p在GC组织中表达下调,lncRNA PVT1的表达水平与GC患者的临床病理特征明显相关,并且与GC患者的预后明显相关.
Abstract
Objective To explore the expression and clinical significance of long noncoding RNA PVT1(lncRNAPVT1)in gastric cancer(GC)tissues.Methods Real-time quantitative fluorescent PCR(RT-qPCR)was used to detect lncRNA PVT1 expression in 196 GC tissues,and the relationship between lncRNA PVT1 expression and clinicopathological features was analyzed by 2 assay.MicroRNA(miRNA,miR)-30a-3p expression in 26 GC tissues was analyzed by t test.Survival analysis was performed by Kaplan-Merier method in 182 GC patients with follow-up data.Results The expression level of lncRNA PVT1 in cancer tissues was significantly higher than that in para-cancer tissues(0.33±0.15 vs.0.28±0.13,t=3.746,P＜0.01).lncRNA PVT1 expression level was correlated with GC tumor size(x2=8.610,P＜0.01),the degree of differentiation of cancer tissue(x2=4.927,P＜0.05),infiltration depth (x2=5.940,P＜0.05),pTNM staging(x2=4.675,P＜0.05)and lymph node metastasis(x2=4.712,P＜0.05).The expression level of miR-30a-3p in GC tissues was significantly decreased as compared with that in para-cancer tissues(0.210±0.156 vs.0.302±0.177,t=2.378,P＜0.05).Survival analysis showed that the overall survival(OS)of patients with high lncRNA PVT1 expression was significantly shor-ter than that of patients with low expression[hazard ratio(HR)=1.746,95％confidence interval(CI):1.154-2.642,P＜0.01].Conclusion The expression of lncRNA PVT1 is up-regulated in GC tissues,and the expression of miR-30a-3p is down-regulated in GC tissues.The expression level of lncRNA PVT1 is significantly correlated with the clinicopathological features and the prognosis of GC patients.
关键词
胃癌/微小RNA/预后Key words
Gastric cancer/MicroRNA/Prognosis引用本文复制引用
基金项目
山西省自然科学基金面上项目(201801D121320)
出版年
2024
NETL
NSTL
万方数据