Expression and clinical significance of long noncoding RNA plasmacytoma variant translocation 1 in gastric cancer
Objective To explore the expression and clinical significance of long noncoding RNA PVT1(lncRNAPVT1)in gastric cancer(GC)tissues.Methods Real-time quantitative fluorescent PCR(RT-qPCR)was used to detect lncRNA PVT1 expression in 196 GC tissues,and the relationship between lncRNA PVT1 expression and clinicopathological features was analyzed by 2 assay.MicroRNA(miRNA,miR)-30a-3p expression in 26 GC tissues was analyzed by t test.Survival analysis was performed by Kaplan-Merier method in 182 GC patients with follow-up data.Results The expression level of lncRNA PVT1 in cancer tissues was significantly higher than that in para-cancer tissues(0.33±0.15 vs.0.28±0.13,t=3.746,P＜0.01).lncRNA PVT1 expression level was correlated with GC tumor size(x2=8.610,P＜0.01),the degree of differentiation of cancer tissue(x2=4.927,P＜0.05),infiltration depth (x2=5.940,P＜0.05),pTNM staging(x2=4.675,P＜0.05)and lymph node metastasis(x2=4.712,P＜0.05).The expression level of miR-30a-3p in GC tissues was significantly decreased as compared with that in para-cancer tissues(0.210±0.156 vs.0.302±0.177,t=2.378,P＜0.05).Survival analysis showed that the overall survival(OS)of patients with high lncRNA PVT1 expression was significantly shor-ter than that of patients with low expression[hazard ratio(HR)=1.746,95％confidence interval(CI):1.154-2.642,P＜0.01].Conclusion The expression of lncRNA PVT1 is up-regulated in GC tissues,and the expression of miR-30a-3p is down-regulated in GC tissues.The expression level of lncRNA PVT1 is significantly correlated with the clinicopathological features and the prognosis of GC patients.
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