Expression and clinical significance of ribosome assembly regulatory factors in colorectal cancer tissue
Objective To explore the expression of ribosomal assembly regulatory factor(PNO1)in colorectal cancer(CRC)and its correlation with clinicopathological features and prognosis through bioin-formatics methods.Methods Download colorectal transcriptome sequencing technology(RNA seq)relat-ed data from the Cancer Genome Atlas(TCGA)database,including 43 normal colorectal samples and 475 colorectal cancer tissue samples.Select the median value of PNO1 expression in colorectal cancer,divide the colorectal cancer patients into high-level and low-level expression groups,and explore the correlation between patient pathological data and PNO1 expression.Apply the Kaplan-Meier method to analyze the difference in survival rate between high-expression and low-expression groups of patients.Apply Cox regres-sion analysis to investigate the impact of PNO1 expression on patient prognosis.Analyze the relevant path-ways in which PNO1 participates in CRC using kyoto encyclopedia of genes and genomes(KEGG)pathway analysis.Results The expression level of PNO1 mRNA in colorectal cancer tissue is significantly higher than that in normal tissue(x2=12.312 P＜0.05).In the TCGA dataset,a comparison of 43 paired sam-ples showed that the expression level of PNO1 in colorectal cancer tissue was significantly higher than that in normal tissue(x2=16.503 P＜0.05).There is no statistically significant difference in PNO1 expression levels among patients in terms of age,site of onset,gender,nerve invasion,vascular invasion,TNM stag-ing,and tumor size(x2=1.730,0.307,0.990,0.990,0.848,0.545,1.806,P＞0.05);There is a statistically significant difference in the expression level of PNO1 in patients with lymph node metastasis,infiltration depth,and differentiation degree(x2=6.275,47.943,11.479,P＜0.05).The Kaplan Meier method was used to analyze the overall survival rate of two groups of patients,and it was found that com-pared with the low expression group of PNO1,the high expression group had a significant decrease in over-all survival rate(x2=11.627 P＜0.05);The OncoLnc database shows that the survival rate of patients with high expression of PNO1 mRNA is lower than that of patients with low expression(x2=17.105 P＜0.05).Univariate Cox regression analysis shows that prognostic factors affecting patients include PNO1 expression,age,degree of differentiation,lymph node metastasis,degree of infiltration,and clinical staging[hazard ratio(HR)=1.284,1.034,1.162,2.021,1.314,2.843;95％confidence interval(CI)=1.101-1.533,1.010-1.055,1.084-1.530,1.556-2.680,1.124-1.628,1.814-4.356,P＜0.05];Multivari-ate Cox regression analysis showed that the independent factors affecting patient prognosis were PNO1 ex-pression,clinical stage,and age(HR=1.290,1.743,1.051;95％CI=1.063-1.614,1.025-2.981,1.023-1.071,P＜0.05).Conclusion PNO1 is highly expressed in CRC tissues,and its expression level is significantly correlated with the clinical pathological characteristics and prognosis of patients.
BioinformaticsColorectal cancerHomo sapiens partner of NOB1 homologPathological features
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